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Antibody induced CD...
Antibody induced CD4 down-modulation of T cells is site-specifically mediated by CD64(+) cells
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- Vogel, Stephanie (författare)
- TWINCORE, Ctr Expt & Clin Infect Res, Inst Expt Infect Res, D-30625 Hannover, Germany.
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- Grabski, Elena (författare)
- TWINCORE, Ctr Expt & Clin Infect Res, Inst Expt Infect Res, D-30625 Hannover, Germany.
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- Buschjaeger, Daniela (författare)
- TWINCORE, Ctr Expt & Clin Infect Res, Inst Expt Infect Res, D-30625 Hannover, Germany.
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- Klawonn, Frank (författare)
- Ostfalia Univ Appl Sci, Dept Comp Sci, D-38302 Wolfenbuttel, Germany.;Helmholtz Ctr Infect Res, Biostat, D-38124 Braunschweig, Germany.
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- Doering, Marius (författare)
- TWINCORE, Ctr Expt & Clin Infect Res, Inst Expt Infect Res, D-30625 Hannover, Germany.;Helmholtz Ctr Infect Res, Biostat, D-38124 Braunschweig, Germany.
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Wang, Junxi (författare)
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- Fletcher, Erika (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Immuneed AB, S-75652 Uppsala, Sweden.
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- Bechmann, Ingo (författare)
- Univ Leipzig, Inst Anat, D-04103 Leipzig, Germany.
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- Witte, Torsten (författare)
- Hannover Med Sch, Clin Immunol & Rheumatol, D-30625 Hannover, Germany.
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- Durisin, Martin (författare)
- Hannover Med Sch, Dept Otolaryngol, D-30625 Hannover, Germany.
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- Schraven, Burkhart (författare)
- Otto Von Guericke Univ, Fac Med, Inst Mol & Clin Immunol, D-39120 Magdeburg, Germany.;Helmholtz Ctr Infect Res, Dept Immune Control, D-38124 Braunschweig, Germany.
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- Mangsbo, Sara M. (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Immuneed AB, S-75652 Uppsala, Sweden.
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- Schoenfeld, Kurt (författare)
- Biotest AG, D-63303 Dreieich, Germany.
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- Czeloth, Niklas (författare)
- Biotest AG, D-63303 Dreieich, Germany.
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- Kalinke, Ulrich (författare)
- TWINCORE, Ctr Expt & Clin Infect Res, Inst Expt Infect Res, D-30625 Hannover, Germany.
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TWINCORE, Ctr Expt & Clin Infect Res, Inst Expt Infect Res, D-30625 Hannover, Germany Ostfalia Univ Appl Sci, Dept Comp Sci, D-38302 Wolfenbuttel, Germany.;Helmholtz Ctr Infect Res, Biostat, D-38124 Braunschweig, Germany. (creator_code:org_t)
- 2015-12-16
- 2015
- Engelska.
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 5
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Treatment of PBMC with the CD4-specific mAb BT-061 induces CD4 down-modulation of T cells. Here we report that addition of BT-061 to purified T cells did not confer this effect, whereas incubation of T cells in BT-061 coated wells restored CD4 down-modulation. These results implied that Fc gamma receptor mediated cell-cell interactions played a role. In consistence with this hypothesis PBMC depleted of CD64(+) monocytes did not confer CD4 down-modulation of BT-061 decorated T cells. Strikingly, CD4 down-modulation was observed in BT-061 treated synovial fluid punctuated from patients' inflamed joints that comprised enhanced numbers of CD64(+) cells. In contrast, in a circulating whole blood system injection of BT-061 did not induce CD4 down-modulation, due to CD64 saturation by serum IgG. Similarly, tonsil derived mononuclear cells devoid of CD64(+) cells did not show CD4 down-modulation, whereas addition of blood derived monocytes restored the effect. Thus, the interaction of BT-061 decorated T cells with CD64(+) cells is needed for CD4 down-modulation, implying that in patients BT-061 would primarily induce CD4 down-modulation at inflammatory sites. These results highlight the need not only to examine the interaction of a given mAb with single Fc gamma R, but also the immunological environment that is appropriate to support such interactions.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
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Till lärosätets databas
- Av författaren/redakt...
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Vogel, Stephanie
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Grabski, Elena
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Buschjaeger, Dan ...
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Klawonn, Frank
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Doering, Marius
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Wang, Junxi
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visa fler...
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Fletcher, Erika
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Bechmann, Ingo
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Witte, Torsten
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Durisin, Martin
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Schraven, Burkha ...
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Mangsbo, Sara M.
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Schoenfeld, Kurt
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Czeloth, Niklas
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Kalinke, Ulrich
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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Uppsala universitet