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Parallel protein de...
Parallel protein detection by solid-phase proximity ligation assay with real-time PCR or sequencing
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- Ebai, Tonge (författare)
- Uppsala universitet,Molekylära verktyg,Ulf Landegren
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- Kamali-Moghaddam, Masood (författare)
- Uppsala universitet,Molekylära verktyg
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- Landegren, Ulf (författare)
- Uppsala universitet,Molekylära verktyg
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(creator_code:org_t)
- 2015-01-05
- 2015
- Engelska.
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Ingår i: Current Protocol in Molecular Biology. - : Wiley. - 1934-3647 .- 1934-3639. ; 109:20
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Proximity ligation assays are a group of protein detection techniques in which reagents with affinity for target proteins, typically antibodies, are coupled to short strands of DNA. DNA-modified affinity reagents are combined in assays constructed such that the coordinated binding of individual target molecules or complexes of interacting proteins by two or more of the reagents, followed by DNA ligation and/or polymerization reactions, gives rise to amplifiable DNA reporter strands. Proximity ligation assays have been shown to exhibit excellent sensitivity in single and multiplexed protein assays for individual or interacting proteins, both in solution and in situ. This unit describes procedures for developing solid-phase proximity ligation assays for soluble proteins using either real-time PCR or DNA sequencing as the readout. In addition, critical steps for assay optimization are discussed.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Annan medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Other Medical Biotechnology (hsv//eng)
Nyckelord
- NGS; PLA probes; PTM; SP-PLA; qPCR
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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