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Zip14 expression in...
Zip14 expression induced by lipopolysaccharides in macrophages attenuates inflammatory response
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- Sayadi, Ahmed (författare)
- Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
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- Nguyen, Anh-Tuan (författare)
- Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
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- Bard, Frederic A (författare)
- Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
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- Bard-Chapeau, Emilie A (författare)
- Institute Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore
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(creator_code:org_t)
- 2012-10-02
- 2013
- Engelska.
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Ingår i: Inflammation Research. - : Springer Science and Business Media LLC. - 1023-3830 .- 1420-908X. ; 62:2
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- OBJECTIVE AND DESIGN: We investigated the role and regulation of zinc transporters in the activation of the inflammatory response in macrophages. Our exploratory computational study found that Zip14 (SLC39A14) was consistently up-regulated in activated macrophages; we therefore focused subsequently on that gene in the mechanistic study.MATERIAL: The expression and function of Zip14 was assessed in primary macrophages obtained by in-vitro differentiation of monocytes from human blood.METHODS: Primary macrophages were subjected to treatments with lipopolysaccharides, cytokines, chemicals, and pharmacological agents. SLC39A14 and inflammatory cytokine gene expressions were assessed by RT-qPCR. Zip14 siRNA knockdown was performed to explore the gene function.RESULTS: Lipopolysaccharide's inflammatory stimulus was a strong inducer of SLC39A14 mRNA expression in macrophages. This induction was dependent on calcium signaling, GC-rich DNA-binding, and NF-κB down-regulation. Impregnation of lipopolysaccharide-stimulated macrophages with the glucocorticoid dexamethasone further enhanced Zip14 expression while reducing interleukin-6 and tumor necrosis factor-α production. Zip14 knockdown in macrophages attenuated the expression and secretion of cytokines, indicating a buffering function for this zinc transporter.CONCLUSIONS: Collectively, our results identified the zinc transporter Zip14 as expressed downstream of lipopolysaccharide signals in macrophages. Zip14 induction had a regulatory function in cytokine production.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
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