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Role of Aromatic Am...
Role of Aromatic Amino Acids in Lipopolysaccharide and Membrane Interactions of Antimicrobial Peptides for use in Plant Disease Control
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- Datta, A. (författare)
- Bose Inst, Dept Biophys, P-1-12 CIT Scheme 7 M, Kolkata 700054, India,Anirban Bhunia`s Group,Bose Institute, Kolkata
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- Bhattacharyya, D. (författare)
- Bose Inst, Dept Biophys, P-1-12 CIT Scheme 7 M, Kolkata 700054, India,Anirban Bhunia`s Group,Bose Institute, Kolkata
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- Singh, Shalini, 1982- (författare)
- Uppsala University,Uppsala universitet,Institutionen för farmaci,Martin Malmsten`s group
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- Ghosh, A. (författare)
- Bose Inst, Dept Biophys, P-1-12 CIT Scheme 7 M, Kolkata 700054, India,Anirban Bhunia,Bose Institute, Kolkata
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- Schmidtchen, Artur (författare)
- Lund University,Lunds universitet,Dermatologi och venereologi, Lund,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Dermatology and Venereology (Lund),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine,Nanyang Technological University
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- Malmsten, Martin (författare)
- Uppsala University,Uppsala universitet,Institutionen för farmaci,Martin Malmsten
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- Bhunia, A. (författare)
- Bose Inst, Dept Biophys, P-1-12 CIT Scheme 7 M, Kolkata 700054, India,Anirban Bhunia,Bose Institute, Kolkata
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(creator_code:org_t)
- 2016
- 2016
- Engelska.
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 291:25, s. 13301-13317
- Relaterad länk:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- KYE28(KYEITTIHNLFRKLTHRLFRRNFGYTLR), the representative sequence of helix D of heparin co-factor II, was demonstrated to be potent against agronomically important Gram-negative plant pathogens X. vesicatoria and X. oryzae,capable of inhibiting disease symptoms in detached tomato leaves. NMR studies in presence of lipopolysaccharide provided structural insights into the mechanisms underlying this, notably in relation to outer membrane permeabilisation. The three-dimensional solution structure of KYE28 in LPS is characterised by a N-ter helical segment, an intermediate loop and an extended C-ter. The two termini are in close proximity to each other via aromatic packing interactions, while the positively charged residues formed an exterior polar shell. To further demonstrate the importance of the aromatic residues for this, a mutant peptide KYE28A, with Ala substitutions at F11, F19, F23 and Y25 showed attenuated antimicrobial activity at high salt concentrations, as well as lower membrane disruption and LPS binding abilities compared to KYE28. In contrast to KYE28, KYE28A adopted an opened out helical structure in LPS with extended N- and C-ter and a small break in between the helical segments. Aromatic packing interactions were completely lost, although hydrophobic interaction between the side chains of hydrophobic residues were still partly retained, imparting an amphipathic character and explaining its residual antimicrobial activity and LPS binding as observed from ellipsometry and ITC. We thus present important structural aspects of KYE28, constituting an aromatic zipper, of potential importance, for the development of novel plant protection agents and therapeutic agents.
Ämnesord
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Nyckelord
- LPS
- Antimicrobial
- Pharmaceutical Physical Chemistry
- Farmaceutisk fysikalisk kemi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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