Sökning: onr:"swepub:oai:DiVA.org:uu-284068" >
Characterization of...
Characterization of preproenkephalin expressing neurons in the nervous system using a transgenic line
-
- Aresh, Bejan, 1984- (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
- Moelijker, Nynke (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
- Blümel, Edda (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
visa fler...
-
- Lagerström, Malin (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
-
visa färre...
-
(creator_code:org_t)
- Engelska.
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Here we have constructed a Cre line to target preproenkephalin expressing cells (Penk-Cre) using a BAC cloning strategy. By crossing our Penk-Cre line with the tdTomato reporter, tdTomato;Penk-Cre expressing cells could be visualized. Penk-Cre was expressed throughout the dorsal spinal cord, where approximately 50% of the neurons were residing within lamina III. Furthermore, single-cell analysis of spinal Penk-Cre expressing cells showed that 41% was positive for Penk mRNA and that a majority (94%) of the population expressed Vglut2 mRNA. Immunohistochemical analysis showed that only 7% and 13% expressed the inhibitory markers Viaat-egfp and Pax2, respectively, hence identifying spinal Penk-Cre expressing neurons as mainly excitatory. The expression of Penk-Cre in the brain was extensive, including dense expression in the striatum, nucleus accumbens and several amygdaloid nuclei. Furthermore, Penk-Cre expression was also shown in insular cortex, cingulated cortex, primary and secondary somatosensory cortices and the trigeminal nuclei. The Penk-Cre line represents a useful genetic tool for future analysis of PENK expressing neurons in the nervous system.
Nyckelord
- characterization
- Cre
- preproenkephalin
- Penk
- Medicinsk vetenskap
- Medical Science
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)