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Inconsistent result...
Inconsistent results in the analysis of ALK rearrangements in non-small cell lung cancer
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- Mattsson, Johanna Sofia Margareta, 1985- (författare)
- Uppsala University,Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke,Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Brunnström, Hans (författare)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine,Skåne University Hospital
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- Jabs, Verena (författare)
- TU Dortmund Univ, Dept Stat, Dortmund, Germany,Department of Statistics, TU Dortmund University, Dortmund, Germany,Technical University of Dortmund
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- Edlund, Karolina (författare)
- Dortmund TU, Leibniz Res Ctr Working Environm & Human Factors, Dortmund, Germany,Leibniz Research Centre for Working Environment and Human Factors (IfADo) at Dortmund TU, Dortmund, Germany,Technical University of Dortmund
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- Jirström, Karin (författare)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Mindus, Stephanie (författare)
- Uppsala University,Uppsala universitet,Lungmedicin och allergologi,Department of Medical Sciences, Respiratory, Allergy and Sleep Research, Uppsala University, Uppsala, Sweden
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- La Fleur, Linnea (författare)
- Uppsala University,Uppsala universitet,Klinisk och experimentell patologi,Science for Life Laboratory, SciLifeLab,Johan Botling,Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Ponten, Fredrik (författare)
- Uppsala University,Uppsala universitet,Klinisk och experimentell patologi,Fredrik Pontén,Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Karlsson, Mats G., 1960- (författare)
- Örebro University,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Research and Education,Univ Orebro, Fac Med & Hlth, Dept Res & Educ, Orebro, Sweden
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- Karlsson, Christina, 1968- (författare)
- Örebro University,Örebro universitet,Institutionen för hälsovetenskaper,Univ Orebro, Sch Hlth Sci, Orebro, Sweden
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- Koyi, Hirsh (författare)
- Karolinska Institutet,Uppsala University,Uppsala universitet,Centrum för klinisk forskning, Gävleborg,Gavle Cent Hosp, Dept Resp Med, Gavle, Sweden,Department of Respiratory Medicine, Gävle hospital, Gävle, Sweden; Centre for Research and Development, Uppsala University, Uppsala, Sweden; County Council of Gävleborg, Gävle, Sweden
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- Brandén, Eva (författare)
- Uppsala University,Uppsala universitet,Centrum för klinisk forskning, Gävleborg,Gavle Cent Hosp, Dept Resp Med, Gavle, Sweden,Department of Respiratory Medicine, Gävle hospital, Gävle, Sweden; Centre for Research and Development, Uppsala University/County Council of Gävleborg, Gävle, Sweden
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- Botling, Johan (författare)
- Uppsala University,Uppsala universitet,Klinisk och experimentell patologi,Johan Botling,Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Helenius, Gisela, 1973- (författare)
- Örebro University,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden,Univ Orebro, Fac Med & Hlth, Dept Lab Med, Orebro, Sweden
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- Micke, Patrick (författare)
- Uppsala University,Uppsala universitet,Klinisk och experimentell patologi,Patrick Micke,Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
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- Svensson, Maria (författare)
- Örebro University
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(creator_code:org_t)
- 2016-08-05
- 2016
- Engelska.
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Ingår i: BMC Cancer. - London, United Kingdom : Springer Science and Business Media LLC. - 1471-2407. ; 16
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://bmccancer.bi...
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https://doi.org/10.1...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://lup.lub.lu.s...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Background: Identification of targetable EML4-ALK fusion proteins has revolutionized the treatment of a minor subgroup of non-small cell lung cancer (NSCLC) patients. Although fluorescence in situ hybridization (FISH) is regarded as the gold standard for detection of ALK rearrangements, ALK immunohistochemistry (IHC) is often used as screening tool in clinical practice. In order to unbiasedly analyze the diagnostic impact of such a screening strategy, we compared ALK IHC with ALK FISH in three large representative Swedish NSCLC cohorts incorporating clinical parameters and gene expression data.Methods: ALK rearrangements were detected using FISH on tissue microarrays (TMAs), including tissue from 851 NSCLC patients. In parallel, ALK protein expression was detected using IHC, applying the antibody clone D5F3 with two different protocols (the FDA approved Ventana CDx assay and our in house Dako IHC protocol). Gene expression microarray data (Affymetrix) was available for 194 patients.Results: ALK rearrangements were detected in 1.7% in the complete cohort and 2.0% in the non-squamous cell carcinoma subgroup. ALK protein expression was observed in 1.9% and 1.5% when applying the Ventana assay or the in house Dako protocol, respectively. The specificity and accuracy of IHC was high (>99%), while the sensitivity was between 69% (Ventana) and 62% (in house Dako protocol). Furthermore, only 67% of the ALK IHC positive cases were positive in both IHC assays. Gene expression analysis revealed that 6/194 (3%) tumors showed high ALK gene expression (≥6AU) and of them only three were positive by either FISH or IHC.Conclusion: The overall frequency of ALK rearrangements based on FISH was lower than previously reported. The sensitivity of both IHC assays was low, and the concordance between the FISH and the IHC assays poor, questioning current strategies to screen with IHC prior to FISH or completely replace FISH by IHC.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Nyckelord
- Anaplastic lymphoma kinase
- non-small cell lung cancer
- immunohistochemistry
- fluorescence in situ hybridization
- Pathology
- Patologi
- Onkologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Mattsson, Johann ...
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Brunnström, Hans
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Jabs, Verena
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Edlund, Karolina
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Jirström, Karin
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Mindus, Stephani ...
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visa fler...
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La Fleur, Linnea
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Ponten, Fredrik
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Karlsson, Mats G ...
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Karlsson, Christ ...
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Koyi, Hirsh
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Brandén, Eva
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Botling, Johan
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Helenius, Gisela ...
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Micke, Patrick
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Svensson, Maria
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Klinisk laborato ...
- Artiklar i publikationen
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BMC Cancer
- Av lärosätet
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Uppsala universitet
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Örebro universitet
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Lunds universitet
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Karolinska Institutet