Performance of Tsol-p27 antigen for the serological diagnosis of cysticercosis in Mozambique

Nhancupe, NoémiaUniv Eduardo Mondlane, Fac Med, Dept Microbiol, Maputo, Mozambique; Karolinska Inst, Microbiol Tumour & Cell Biol, Stockholm, Sweden (författare)

The diagnosis of neurocysticercosis (NCC) requires expensive neuroimaging techniques that are seldom affordable for people in endemic countries. Accordingly, there is a need for new low-cost diagnostic methods that offer high sensitivity and specificity. In this study, we evaluated Western blot analysis of the previously described recombinant antigen Tsol-p27 in relation to a commercial or in-house enzyme-linked immunosorbent assay (ELISA) for NCC, and compared the results with those provided by a commercial enzyme-linked immunoelectrotransfer blot (EITB) assay, which was regarded as the reference standard method. The analysed serum samples were obtained from 165 people, 18 of whom were confirmed to be NCC positive by EITB. Comparing our Western blot analysis of Tsol-p27 with a previous evaluation performed in Central America showed similar specificity (96.69% versus 97.8%) and sensitivity (85.71% versus 86.7%). The present results indicate that the recombinant Tsol-p27 antigen provides good sensitivity and specificity, and might be preferable as a diagnostic antigen in poorly equipped laboratories in endemic countries.

Noormahomed, E. V.Univ Eduardo Mondlane, Fac Med, Dept Microbiol, Maputo, Mozambique.;Univ Calif San Diego, Dept Med, San Diego, CA 92103 USA.;Univ Lurio, Fac Ciencias Saude, Nampula, Mozambique. (författare)
Afonso, S.Univ Eduardo Mondlane, Fac Vet, Dept Paraclin, Maputo, Mozambique. (författare)
Falk, K. I.Karolinska Institutet,Karolinska Inst, Microbiol Tumour & Cell Biol, Stockholm, Sweden.;Publ Hlth Agcy Sweden, Solna, Sweden. (författare)
Lindh, JohanUppsala universitet,Mikrobiologi(Swepub:uu)johli354 (författare)
Univ Eduardo Mondlane, Fac Med, Dept Microbiol, Maputo, Mozambique; Karolinska Inst, Microbiol Tumour & Cell Biol, Stockholm, SwedenUniv Eduardo Mondlane, Fac Med, Dept Microbiol, Maputo, Mozambique.;Univ Calif San Diego, Dept Med, San Diego, CA 92103 USA.;Univ Lurio, Fac Ciencias Saude, Nampula, Mozambique. (creator_code:org_t)

Ingår i:Journal of Helminthology: Cambridge University Press90:5, s. 630-6330022-149X1475-2697

Av författaren/redakt...: Nhancupe, Noémia; Noormahomed, E. ...; Afonso, S.; Falk, K. I.; Lindh, Johan

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Infektionsmedici ...

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