Sökning: onr:"swepub:oai:DiVA.org:uu-307265" >
Analysis of the bra...
Analysis of the brain mural cell transcriptome
-
- He, Liqun (författare)
- Uppsala universitet,Vaskulärbiologi
-
- Vanlandewijck, Michael (författare)
- Karolinska Institutet,Uppsala universitet,Vaskulärbiologi,Karolinska Inst, ICMC, SE-14157 Huddinge, Sweden.
-
- Raschperger, Elisabeth (författare)
- Uppsala universitet,Vaskulärbiologi,Karolinska Inst, ICMC, SE-14157 Huddinge, Sweden.
-
visa fler...
-
- Mae, Maarja Andaloussi (författare)
- Uppsala universitet,Vaskulärbiologi
-
- Jung, Bongnam (författare)
- Uppsala universitet,Vaskulärbiologi
-
- Lebouvier, Thibaud (författare)
- Uppsala universitet,Vaskulärbiologi
-
- Ando, Koji (författare)
- Uppsala universitet,Vaskulärbiologi
-
- Hofmann, Jennifer (författare)
- Uppsala universitet,Vaskulärbiologi
-
- Keller, Annika (författare)
- Univ Zurich, Zurich Univ Hosp, Div Neurosurg, Zurich, Switzerland.
-
- Betsholtz, Christer (författare)
- Karolinska Institutet,Uppsala universitet,Vaskulärbiologi,Karolinska Inst, ICMC, SE-14157 Huddinge, Sweden.
-
visa färre...
-
(creator_code:org_t)
- 2016-10-11
- 2016
- Engelska.
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
- Relaterad länk:
-
https://uu.diva-port... (primary) (Raw object)
-
visa fler...
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Pericytes, the mural cells of blood microvessels, regulate microvascular development and function and have been implicated in many brain diseases. However, due to a paucity of defining markers, pericyte identification and functional characterization remain ambiguous and data interpretation problematic. In mice carrying two transgenic reporters, Pdgfrb-eGFP and NG2-DsRed, we found that double-positive cells were vascular mural cells, while the single reporters marked additional, but non-overlapping, neuroglial cells. Double-positive cells were isolated by fluorescence-activated cell sorting (FACS) and analyzed by RNA sequencing. To reveal defining patterns of mural cell transcripts, we compared the RNA sequencing data with data from four previously published studies. The meta-analysis provided a conservative catalogue of 260 brain mural cell-enriched gene transcripts. We validated pericyte-specific expression of two novel markers, vitronectin (Vtn) and interferon-induced transmembrane protein 1 (Ifitm1), using fluorescent in situ hybridization and immunohistochemistry. We further analyzed signaling pathways and interaction networks of the pericyte-enriched genes in silico. This work provides novel insight into the molecular composition of brain mural cells. The reported gene catalogue facilitates identification of brain pericytes by providing numerous new candidate marker genes and is a rich source for new hypotheses for future studies of brain mural cell physiology and pathophysiology.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
He, Liqun
-
Vanlandewijck, M ...
-
Raschperger, Eli ...
-
Mae, Maarja Anda ...
-
Jung, Bongnam
-
Lebouvier, Thiba ...
-
visa fler...
-
Ando, Koji
-
Hofmann, Jennife ...
-
Keller, Annika
-
Betsholtz, Chris ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Medicinsk biotek ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Kardiologi
- Artiklar i publikationen
-
Scientific Repor ...
- Av lärosätet
-
Uppsala universitet
-
Karolinska Institutet