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Characterization of...
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Cocco, AriannaUppsala universitet,Fysiologi
(författare)
Characterization of the gamma-aminobutyric acid signaling system in the zebrafish (danio rerio hamilton) central nervous system by reverse transcription-quantitative polymerase chain reaction
- Artikel/kapitelEngelska2017
Förlag, utgivningsår, omfång ...
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Elsevier BV,2017
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-317583
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-317583URI
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https://doi.org/10.1016/j.neuroscience.2016.07.018DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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In the vertebrate brain, inhibition is largely mediated by raminobutyric acid (GABA). This neurotransmitter comprises a signaling machinery of GABA(A), GABA(B) receptors, transporters, glutamate decarboxylases (gads) and 4-aminobutyrate aminotransferase (abat), and associated proteins. Chloride is intimately related to GABAA receptor conductance, GABA uptake, and GADs activity. The response of target neurons to GABA stimuli is shaped by chloride-cation co-transporters (CCCs), which strictly control Cl- gradient across plasma membranes. This research profiled the expression of forty genes involved in GABA signaling in the zebrafish (Danio rerio) brain, grouped brain regions and retinas. Primer pairs were developed for reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The mRNA levels of the zebrafish GABA system share similarities with that of mammals, and confirm previous studies in non-mammalian species. Proposed GABAA receptors are alpha(1)beta(2)gamma(2), alpha(1)beta(2)delta, alpha(2b)beta(3), alpha(2b)beta(3)delta, alpha(4)beta(2)gamma(2), alpha(4)beta(2)gamma, alpha(6b)beta(2)gamma(2) and alpha(6b)beta(2)delta. Regional brain differences were documented. Retinal hetero- or homomeric rho-composed GABAA receptors could exist, accompanying alpha(1)beta(y)gamma(2), alpha(1)beta(y)delta, alpha(6a)beta(y)gamma(2,) alpha(6a)beta(y)delta. Expression patterns of alpha(6a) and alpha(6b) were opposite, with the former being more abundant in retinas, the latter in brains. Given the stoichiometry alpha(6w)beta(y)gamma(z), alpha(6a-) or alpha(6b)-containing receptors likely have different regulatory mechanisms. Different gene isoforms could originate after the rounds of genome duplication during teleost evolution. This research depicts that one isoform is generally more abundantly expressed than the other. Such observations also apply to GABAB receptors, GABA transporters, GABA-related enzymes, CCCs and GABAA receptor associated proteins, whose presence further strengthens the proof of a GABA system in zebrafish.
Ämnesord och genrebeteckningar
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GABA
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comparative neuroscience
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teleost
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zebrafish
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neurotransmitter systems
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receptors
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Rönnberg, A. M. CarolinaUppsala universitet,Institutionen för neurovetenskap
(författare)
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Jin, ZheUppsala universitet,Fysiologi(Swepub:uu)zheji315
(författare)
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André, Goncalo IgrejaUppsala universitet,Institutionen för neurovetenskap,Univ Western Australia, Ctr Evolutionary Biol, 35 Stirling Hwy, Crawley, WA 6009, Australia.
(författare)
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Vossen, Laura E.Uppsala universitet,Fysiologi(Swepub:uu)lauvo179
(författare)
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Bhandage, Amol K.Uppsala universitet,Fysiologi(Swepub:uu)amebh673
(författare)
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Thörnqvist, Per-OveUppsala universitet,Fysiologi(Swepub:uu)perothor
(författare)
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Birnir, BryndisUppsala universitet,Fysiologi(Swepub:uu)brybi979
(författare)
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Winberg, SvanteUppsala universitet,Fysiologi(Swepub:uu)svanwinb
(författare)
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Uppsala universitetFysiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Neuroscience: Elsevier BV343, s. 300-3210306-45221873-7544
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