Sökning: onr:"swepub:oai:DiVA.org:uu-326158" >
Affinity Proteomics...
Affinity Proteomics Applied to Patient CSF Identifies Protein Profiles Associated with Neuropathic Pain and Fibromyalgia
-
- Lind, Anne-Li (författare)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper
-
- Just, David (författare)
- SciLifeLab, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden,Affinity Proteomics
-
- Mikus, Maria (författare)
- SciLifeLab, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden,Affinity Proteomics
-
visa fler...
-
- Fredolini, Claudia (författare)
- SciLifeLab, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden,Affinity Proteomics
-
- Ioannou, Marina (författare)
- SciLifeLab, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden,Affinity Proteomics
-
- Gerdle, Björn (författare)
- Linköping University, Linköping, Sweden,Pain and Rehabilitation Center, and Department of Medical and Health Sciences,
-
- Bäckryd, Emmanuel (författare)
- Linköping University, Linköping, Sweden,Pain and Rehabilitation Center, and Department of Medical and Health Sciences,
-
- Tanum, Lars (författare)
- Akershus University Hospital, Lørenskog, Norway,Department of R&D in Mental Health
-
- Gordh, Torsten (författare)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper
-
- Nilsson, Peter (författare)
- SciLifeLab, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden,Affinity Proteomics
-
- Månberg, Anna (författare)
- SciLifeLab, School of Biotechnology, KTH – Royal Institute of Technology, Stockholm, Sweden,Affinity Proteomics
-
visa färre...
-
(creator_code:org_t)
- Engelska.
- Relaterad länk:
-
https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Objective: Today, there are no biological tests on which to base pain diagnoses, treatment choices or to understand the biological processes underlying and accompanying chronic pain for the individual pain patient. Relevant biological markers would greatly aid in diagnosis and treatment of patients with chronic pain. Our study aimed to find proteins in CSF associated with fibromyalgia and neuropathic pain, two common and poorly understood chronic pain conditions.Methods: We have performed CSF protein profiling of 55 proteins using a 100-plex antibody suspension bead array. We collected, analyzed and compared CSF samples from 25 patients with neuropathic pain (two independent sets, n=14 patients for discovery and n=11 for verification), 40 patients with fibromyalgia and 135 controls without neurological disease from two different populations.Results: We found significant differences in CSF protein levels between patients and controls (p<0.05). Among these proteins, Apolipoprotein C1 (APOC1) was found to be increased in CSF of neuropathic pain patients compared to controls and there was a non-significant trend for increased levels also in fibromyalgia patient CSF. Ectonucleotide pyrophosphatase (ENPP2, Autotaxin) was increased in the CSF of fibromyalgia patients compared to all other groups including neuropathic pain patients. Multivariate analysis revealed partially overlapping and partially distinct CSF profiles in neuropathic pain patients compared with fibromyalgia and controls for several other proteins including angiotensinogen (AGT), prostaglandin-H2 D-isomerase (PTGDS), neurexin-1 (NRXN1), superoxide dismutase 1 (SOD1) and superoxide dismutase 3 (SOD3).Conclusions: Our results, suggest that the CSF protein profiles of neuropathic pain and fibromyalgia patients may be different from each other and from those of controls. CSF levels of APOC1, ENPP2, AGT, PTGDS, NRXN1, SOD1 and SOD3 should be further investigated for their potential to serve as biomarkers of different kinds of pain pathophysiology.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Anestesi och intensivvård (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Anesthesiology and Intensive Care (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- cerebrospinal fluid
- biomarker
- human
- chronic pain
- neuropathic pain
- fibromyalgia
- spinal cord stimulation
- mechanism of action
- radiculopathy
- protein
- inflammation
- neuroinflammation
- mass spectrometry
- antibody suspension bead array
- protein profiling
- Anaesthesiology and Intensive Care
- Anestesiologi och intensivvård
- Bioinformatik
- Bioinformatics
- Biokemi
- Biochemistry
- Biology with specialization in Molecular Biology
- Biologi med inriktning mot molekylärbiologi
- Biomedicinsk laboratorievetenskap
- Biomedical Laboratory Science
- Kemi med inriktning mot analytisk kemi
- Chemistry with specialization in Analytical Chemistry
- Klinisk kemi
- Clinical Chemistry
- Medical Science
- Medicinsk vetenskap
- Molekylär medicin
- Molecular Medicine
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)
- Av författaren/redakt...
-
Lind, Anne-Li
-
Just, David
-
Mikus, Maria
-
Fredolini, Claud ...
-
Ioannou, Marina
-
Gerdle, Björn
-
visa fler...
-
Bäckryd, Emmanue ...
-
Tanum, Lars
-
Gordh, Torsten
-
Nilsson, Peter
-
Månberg, Anna
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Neurovetenskaper
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Klinisk laborato ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Hälsovetenskap
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Anestesi och int ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Biomedicinsk lab ...
- Av lärosätet
-
Uppsala universitet