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Intervention of rib...
Intervention of ribosomal RNA in HET-s prion aggregation Intervention of ribosomal RNA in HET-s prion aggregation
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- Pang, Yanhong (författare)
- Uppsala universitet,Struktur- och molekylärbiologi
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- Kovachev, Petar Stefanov (författare)
- Uppsala universitet,Struktur- och molekylärbiologi
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- Macedo, Bruno (författare)
- Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, Bloco B, Subsolo, Sala 17, Rio de Janeiro, Brazil
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visa fler...
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- Westermark, Gunilla (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Sanyal, Suparna (författare)
- Uppsala universitet,Struktur- och molekylärbiologi
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- The role of nucleic acids in prion aggregation / disaggregation has remained unclear. Here, using HET-s prion from Podospora anserina as a model system, we have studied the role of RNA, particularly different domains of ribosomal RNA, in its aggregation process. Our results show that domain V rRNA, from the large subunit of the ribosome, substantially prevents amyloid aggregation of the HET-s prion in a concentration dependent manner. Instead, it promotes the formation of oligomeric seeds, which facilitate de novo HET-s aggregation. The interaction sites for the HET-s prion on domain V rRNA were also identified and shown to overlap with the sites previously found to responsible for the protein folding activity of the ribosome (PFAR). This study provides a missing link between the role of rRNA-based PFAR and prion propagation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Molekylär cellbiologi
- Molecular Cellbiology
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)