Sökning: onr:"swepub:oai:DiVA.org:uu-339724" >
Multicenter Assessm...
Multicenter Assessment of Animal-free Collagenase AF-1 for Human Islet Isolation
-
- Brandhorst, Daniel (författare)
- Univ Oxford, Nuffield Dept Surg Sci, Oxford, England.;Churchill Hosp, OCDEM, Oxford, England.
-
- Parnaud, Geraldine (författare)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
-
- Friberg, Andrew, 1979- (författare)
- Uppsala universitet,Klinisk immunologi
-
visa fler...
-
- Lavallard, Vanessa (författare)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
-
- Demuylder-Mischler, Sandrine (författare)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
-
- Hughes, Stephen (författare)
- Univ Oxford, Nuffield Dept Surg Sci, Oxford, England.;Churchill Hosp, OCDEM, Oxford, England.
-
- Saphoerster, Julia (författare)
- SERVA Electrophoresis GmbH, Uetersen, Germany.
-
- Kurfuerst, Manfred (författare)
- SERVA Electrophoresis GmbH, Uetersen, Germany.
-
- Korsgren, Olle (författare)
- Uppsala universitet,Klinisk immunologi
-
- Berney, Thierry (författare)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
-
- Johnson, Paul R. V. (författare)
- Univ Oxford, Nuffield Dept Surg Sci, Oxford, England.;Churchill Hosp, OCDEM, Oxford, England.
-
visa färre...
-
Univ Oxford, Nuffield Dept Surg Sci, Oxford, England;Churchill Hosp, OCDEM, Oxford, England. Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland. (creator_code:org_t)
- 2017-12-18
- 2017
- Engelska.
-
Ingår i: Cell Transplantation. - : Sage Publications. - 0963-6897 .- 1555-3892. ; 26:10, s. 1688-1693
- Relaterad länk:
-
https://uu.diva-port... (primary) (Raw object)
-
visa fler...
-
https://journals.sag...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have recently become available for human islet isolation. This report describes the initial experiences of 3 different islet transplant centers. Thirty-four human pancreases were digested using 1 vial of the 6 different lots of Collagenase AF-1 (2,000-2,583 PZ-U/vial) supplemented with 4 different lots of NP AF in a range of 50 to 160 DMC-U per pancreas. Isolation, culture, and quality assessment were performed using standard techniques as previously described. All data are presented as mean +/- standard error of the mean (SEM). Variability of pancreas weight was associated with a wide range of collagenase and NP activities, ranging from 12.7 to 46.6 PZ-U/g (26.0 +/- 1.5 PZ-U/g) and 0.4 to 3.0 DMC-U/g (1.5 +/- 0.1 DMC-U/g), respectively. Postpurification islet yield was 296,494 +/- 33,620 islet equivalents (IEQ) equivalent to 3,274 +/- 450 IEQ/g with a purity of 55.9% +/- 3.2%. Quality assessment performed after 2 to 4 d of culture demonstrated a viability of 88.1% +/- 1.5% and a stimulation index of 3.7 +/- 0.7. Eighteen of the 34 preparations were transplanted into type 1 diabetic patients equivalent to a transplantation rate of 52.9%. Six preparations, which were infused into patients as first transplant, could be analyzed and increased the fasting C-peptide level from 0.11 +/- 0.08 pretransplant to 1.23 +/- 0.24 and 2.27 +/- 0.31 ng/mL 3 and 6 mo posttransplant (P < 0.05), respectively. Insulin requirements were simultaneously reduced at the same time from 39.2 +/- 3.8 IU/d before transplantation to 10.8 +/- 4.1 and 4.0 +/- 2.3 IU/d, after 3 and 6 mo posttransplant (P < 0.05), respectively. This study demonstrates the efficiency of AF SERVA Collagenase AF-1 and NP AF for clinical islet isolation and transplantation. The new plant-based production process makes these products a safe new option for the islet field.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- human islet isolation
- human islet transplantation
- collagenase
- islet quality assessment
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Brandhorst, Dani ...
-
Parnaud, Geraldi ...
-
Friberg, Andrew, ...
-
Lavallard, Vanes ...
-
Demuylder-Mischl ...
-
Hughes, Stephen
-
visa fler...
-
Saphoerster, Jul ...
-
Kurfuerst, Manfr ...
-
Korsgren, Olle
-
Berney, Thierry
-
Johnson, Paul R. ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Endokrinologi oc ...
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Cellbiologi
- Artiklar i publikationen
-
Cell Transplanta ...
- Av lärosätet
-
Uppsala universitet