Sökning: onr:"swepub:oai:DiVA.org:uu-339802" >
Alpha-synuclein oli...
Alpha-synuclein oligomer-selective antibodies reduce intracellular accumulation and mitochondrial impairment in alpha-synuclein exposed astrocytes
-
- Gustafsson, Gabriel (författare)
- Uppsala universitet,Geriatrik
-
- Lindström, Veronica (författare)
- Uppsala universitet,Geriatrik
-
- Rostami, Jinar (författare)
- Uppsala universitet,Geriatrik
-
visa fler...
-
- Nordström, Eva (författare)
- BioArct AB, Stockholm
-
- Lannfelt, Lars (författare)
- Uppsala universitet,Geriatrik
-
- Bergström, Joakim (författare)
- Uppsala universitet,Geriatrik
-
- Ingelsson, Martin (författare)
- Uppsala universitet,Geriatrik
-
- Erlandsson, Anna (författare)
- Uppsala universitet,Geriatrik
-
visa färre...
-
(creator_code:org_t)
- 2017-12-11
- 2017
- Engelska.
-
Ingår i: Journal of Neuroinflammation. - : Springer Science and Business Media LLC. - 1742-2094. ; 14
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://jneuroinflam...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: Due to its neurotoxic properties, oligomeric alpha-synuclein (α-syn) has been suggested as an attractive target for passive immunization against Parkinson’s disease (PD). In mouse models of PD, antibody treatment has been shown to lower the levels of pathogenic α-syn species, including oligomers, although the mechanisms of action remain unknown. We have previously shown that astrocytes rapidly engulf α-syn oligomers that are intracellularly stored, rather than degraded, resulting in impaired mitochondria.Methods: The aim of the present study was to investigate if the accumulation of α-syn in astrocytes can be affected by α-syn oligomer-selective antibodies. Co-cultures of astrocytes, neurons, and oligodendrocytes were derived from embryonic mouse cortex and exposed to α-syn oligomers or oligomers pre-incubated with oligomer-selective antibodies.Results: In the presence of antibodies, the astrocytes displayed an increased clearance of the exogenously added α-syn, and consequently, the α-syn accumulation in the culture was markedly reduced. Moreover, the addition of antibodies rescued the astrocytes from the oligomer-induced mitochondrial impairment.Conclusions: Our results demonstrate that oligomer-selective antibodies can prevent α-syn accumulation and mitochondrial dysfunction in cultured astrocytes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Geriatrik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Geriatrics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- alpha-synuclein oligomers
- Astrocytes
- Antibodies
- Mitochondria
- Lysosomal degradation
- Parkinson's disease
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas