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Synthetic green flu...
Synthetic green fluorescent protein (GFP) chromophore analog for rapid, selective and sensitive detection of cyanide in water and in living cells
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- Mirajkar, Abhishek L. (författare)
- Univ Mumbai, Dept Chem, Bombay 400098, Maharashtra, India
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- Mittapelli, Lavanya L. (författare)
- Univ Mumbai, Dept Chem, Bombay 400098, Maharashtra, India
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- Nawale, Ganesh N. (författare)
- Uppsala universitet,Polymerkemi
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- Gorea, Kiran R. (författare)
- Univ Mumbai, Dept Chem, Bombay 400098, Maharashtra, India
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(creator_code:org_t)
- ELSEVIER SCIENCE SA, 2018
- 2018
- Engelska.
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Ingår i: Sensors and actuators. B, Chemical. - : ELSEVIER SCIENCE SA. - 0925-4005 .- 1873-3077. ; 265, s. 257-263
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Here, we report Green Fluorescent Protein (GFP) chromophore analog as a turn-on fluorescent chemodosimeter (THBI) for selective detection of cyanide in water, on solid state and in living cells. The detection limit was found to be 0.17 mu M (4.5 ppb). The time dependent study revealed that there is a rapid enhancement in fluorescence intensity (in less than 5s) and was constant over the period of 1 h. Cell imaging data exhibited that THBI was successfully crossed cell membrane and visualized fluorescence response in live HCT cells.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- GFP
- HBI
- THBI
- Cyanide chemodosimeter
- Fluorogenic chemosensor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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