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Expression of an ac...
Expression of an active and inactive clip-domain Serine Protease Isolated from Signal Crayfish in an insect cell line
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Korkut, Gül Gizem (författare)
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- Sirikharin, Ratchanok (författare)
- Söderhäll
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- Noonin, Chadanat (författare)
- Söderhäll
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visa fler...
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- Söderhäll, Kenneth (författare)
- Science for Life Laboratory,Söderhäll
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- SöderhällI, Irene (författare)
- Science for Life Laboratory,Söderhäll
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- In this study, we aimed to elucidate the cleavage specificity and endogenous substrate of a clip-domain serine protease from the crayfish Pacifastacus leniusculus (PlcSP). This serine protease can bind to white spot syndrome virus (WSSV) and is important for the entry of WSSV into the hematopoietic tissue (HPT). In order to understand its intrinsic role in the serine protease cascade, we wanted to use substrate phage display method to clarify cleavage specificity of PlcSP. Firstly, we aimed to make recombinant active and inactive forms of this PlcSP using human embryonic kidney cell line (HEK293) however PlcSP appeared to be toxic for mammalian cells and we were not able to express this crustacean protein. However, we were able to express these recombinantPlcSP both in their zymogenic and active forms in Sf9 insect cell line.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Serine protease
- Pacifastacus leniusculus
- invertebrate immunity
- Sf9
- substrate phage display
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)