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Amperometric Self-R...
Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection
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- Campos-Beltran, Diana (författare)
- Univ Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, Germany
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- Konradsson-Geuken, Åsa (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden
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- Quintero, Jorge E. (författare)
- Univ Kentucky, CenMeT, Lexington, KY 40506 USA;Quanteon LLC, Nicholasville, KY 40356 USA
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- Marshall, Lisa (författare)
- Univ Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, Germany
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(creator_code:org_t)
- 2018-03-06
- 2018
- Engelska.
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Ingår i: Biosensors. - : MDPI. - 2079-6374. ; 8:1
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 +/- 0.83 pA/mu M to D-serine, a limit of detection (LOD) of 0.17 +/- 0.01 mu M, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean +/- SEM; n = 12) that can be used for freely moving studies.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- D-serine
- biosensor
- microelectrode array
- amperometry
- self-referencing
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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