Sökning: onr:"swepub:oai:DiVA.org:uu-361399" > Avidin-biotin cross...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03376naa a2200373 4500 | |
001 | oai:DiVA.org:uu-361399 | |
003 | SwePub | |
008 | 180924s2018 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3613992 URI |
024 | 7 | a https://doi.org/10.1021/acs.biomac.8b014842 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Nyström, Linau Uppsala universitet,Institutionen för farmaci4 aut0 (Swepub:uu)linny502 |
245 | 1 0 | a Avidin-biotin cross-linked microgel multilayers as carriers for antimicrobial peptides |
264 | c 2018-11-14 | |
264 | 1 | b American Chemical Society (ACS),c 2018 |
338 | a print2 rdacarrier | |
520 | a Herein, we report on the formation of cross-linked antimicrobial peptide-loaded microgel multilayers. Poly(ethyl acrylate- co-methacrylic acid) microgels were synthesized and functionalized with biotin to enable the formation of microgel multilayers cross-linked with avidin. Microgel functionalization and avidin cross-linking were verified with infrared spectroscopy, dynamic light scattering, and z-potential measurements, while multilayer formation (up to four layers) was studied with null ellipsometry and quartz crystal microbalance with dissipation (QCM-D). Incorporation of the antimicrobial peptide KYE28 (KYEITTIHNLFRKLTHRLFRRNFGYTLR) into the microgel multilayers was achieved either in one shot after multilayer formation or through addition after each microgel layer deposition. The latter was found to strongly promote peptide incorporation. Further, antimicrobial properties of the peptide-loaded microgel multilayers against Escherichia coli were investigated and compared to those of a peptide-loaded microgel monolayer. Results showed a more pronounced suppression in bacterial viability in suspension for the microgel multilayers. Correspondingly, LIVE/DEAD staining showed promoted disruption of adhered bacteria for the KYE28-loaded multilayers. Taken together, cross-linked microgel multilayers thus show promise as high load surface coatings for antimicrobial peptides. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmaceutiska vetenskaper0 (SwePub)301012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmaceutical Sciences0 (SwePub)301012 hsv//eng |
653 | a Pharmaceutical Physical Chemistry | |
653 | a Farmaceutisk fysikalisk kemi | |
700 | 1 | a Al-Rammahi, Nooru Uppsala universitet,Institutionen för farmaci4 aut |
700 | 1 | a Malekkhaiat Häffner, Sarau Uppsala universitet,Institutionen för farmaci4 aut0 (Swepub:uu)sarma170 |
700 | 1 | a Strömstedt, Adam A.,c Dr,d 1977-u Uppsala universitet,Farmakognosi4 aut0 (Swepub:uu)adstr021 |
700 | 1 | a Browning, Kathryn L.u Uppsala universitet,Institutionen för farmaci4 aut0 (Swepub:uu)katbr474 |
700 | 1 | a Malmsten, Martinu Uppsala universitet,Institutionen för farmaci4 aut0 (Swepub:uu)mamal991 |
710 | 2 | a Uppsala universitetb Institutionen för farmaci4 org |
773 | 0 | t Biomacromoleculesd : American Chemical Society (ACS)g 19:12, s. 4691-4702q 19:12<4691-4702x 1525-7797x 1526-4602 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-361399 |
856 | 4 8 | u https://doi.org/10.1021/acs.biomac.8b01484 |
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