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Mouse Mast Cell Pro...
Mouse Mast Cell Protease 4 Deletion Protects Heart Function and Survival After Permanent Myocardial Infarction
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- Houde, Martin (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Pharmacol Physiol, Sherbrooke, PQ, Canada; Leiden Univ, Leiden Acad, Ctr Drug Res, Div BioTherapeut, Leiden, Netherlands
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- Schwertani, Adel (författare)
- McGill Univ, Dept Med, Montreal, PQ, Canada
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- Touil, Hanene (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Pharmacol Physiol, Sherbrooke, PQ, Canada
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- Desbiens, Louisane (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Pharmacol Physiol, Sherbrooke, PQ, Canada
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- Sarrhini, Otman (författare)
- Univ Sherbrooke, CRCHUS, Sherbrooke Mol Imaging Ctr, Dept Nucl Med & Radiobiol, Sherbrooke, PQ, Canada
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- Lecomte, Roger (författare)
- Univ Sherbrooke, CRCHUS, Sherbrooke Mol Imaging Ctr, Dept Nucl Med & Radiobiol, Sherbrooke, PQ, Canada
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- Lepage, Martin (författare)
- Univ Sherbrooke, CRCHUS, Sherbrooke Mol Imaging Ctr, Dept Nucl Med & Radiobiol, Sherbrooke, PQ, Canada
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- Gagnon, Hugo (författare)
- PhenoSwitch Biosci Inc, Sherbrooke, PQ, Canada
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- Takai, Shinji (författare)
- Osaka Med Coll, Dept Innovat Med, Osaka, Japan
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- Pejler, Gunnar (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Uppsala, Sweden,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University
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- Jacques, Danielle (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Anat & Cell Biol, Sherbrooke, PQ, Canada
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- Gobeil, Fernand, Jr. (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Pharmacol Physiol, Sherbrooke, PQ, Canada
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- Day, Robert (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Surg, Sherbrooke, PQ, Canada
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- D'Orleans-Juste, Pedro (författare)
- Univ Sherbrooke, Fac Med & Sci Sante, Dept Pharmacol Physiol, Sherbrooke, PQ, Canada
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(creator_code:org_t)
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- 2018-08-31
- 2018
- Engelska.
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Ingår i: Frontiers in Pharmacology. - : Frontiers Media SA. - 1663-9812. ; 9
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- Chymase, a mast cell serine protease involved in the generation of multiple cardiovascular factors, such as angiotensin II and endothelin-1 (ET-1), is elevated and participates in tissue degeneration after permanent myocardial infarction (PMI). Anesthetized 4-month old male wild-type (WT) C57BL/6J mice and mouse mast cell protease-4 knockout (mMCP-4 KO) congeners were subjected to ligation of the left anterior descending (LAD) coronary artery. A group of mice was then subjected to Kaplan-Meier 28-day survival analysis. In another group of mice, F-18-fluorodeoxyglucose positron emission tomography (PET) was performed to evaluate heart function and the infarcted zone 3 days post-PMI surgery. Cardiac morphology following PMI was evaluated on formalin-fixed heart slices and glycoproteomic analysis was performed using mass spectrometry. Finally, cardiac and lung tissue content of immunoreactive ET-1 was determined. PMI caused 60% mortality in WT mice, due to left ventricular wall rupture, and 7% in mMCP-4 KO mice. Cardiac PET analysis revealed a significant reduction in left ventricular volume (systolic and diastolic) and preserved the ejection fraction in mMCP-4 KO compared to WT animals. The infarcted area, apoptotic signaling and wall remodeling were significantly decreased in mMCP-4 KO mice compared to their WT congeners, while collagen deposition was increased. Glycoproteomic analysis showed an increase in apolipoprotein A1, an established chymase substrate in mMCP-4 KO mice compared to WT mice post-PMI. ET-1 levels were increased in the lungs of WT, but not mMCP-4 KO mice, 24 h post-PMI. Thus, the genetic deletion of mMCP-4 improved survival and heart function post-PMI.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- chymase
- mouse mast cell protease 4
- myocardial infarction
- positron emission tomography
- glycoproteomics
- active caspase-3
- collagen
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Houde, Martin
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Schwertani, Adel
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Touil, Hanene
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Desbiens, Louisa ...
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Sarrhini, Otman
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Lecomte, Roger
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Lepage, Martin
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Gagnon, Hugo
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Takai, Shinji
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Pejler, Gunnar
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Jacques, Daniell ...
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Gobeil, Fernand, ...
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Day, Robert
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D'Orleans-Juste, ...
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