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Binding site differ...
Binding site differences revealed by crystal structures of Plasmodium falciparum and bovine acyl-CoA binding protein
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van Aalten, DMF (författare)
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Milne, KG (författare)
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- Zou, JY (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Strukturell molekylärbiologi
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visa fler...
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- Kleywegt, GJ (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Strukturell molekylärbiologi
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- Bergfors, T (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Strukturell molekylärbiologi
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Ferguson, MAJ (författare)
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Knudsen, J (författare)
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- Jones, TA (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Strukturell molekylärbiologi
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visa färre...
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(creator_code:org_t)
- 2001
- 2001
- Engelska.
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Ingår i: JOURNAL OF MOLECULAR BIOLOGY. - 0022-2836. ; 309:1, s. 181-192
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA molecules in the cell and plays a role in fatty acid metabolism. The biochemical properties of Plasmodium falciparum ACBP are described together with the 2.0 A resolution crystal structures of a P. falciparum ACBP-acyl-CoA complex and of bovine ACBP in two crystal forms. Overall, the bovine ACBP crystal structures are similar to the NMR structures published previously; however, the bovine and parasite ACBP structures are less similar. The parasite ACBP is shown to have a different ligand-binding pocket, leading to an acyl-CoA binding specificity different from that of bovine ACBP. Several non-conservative differences in residues that interact with the ligand were identified between the mammalian and parasite ACBPs. These, together with measured binding-specificity differences, suggest that there is a potential for the design of molecules that might selectively block the acyl-CoA binding site.
Nyckelord
- acyl-CoA; structure; Plasmodium falciparum; drug design; X-ray crystallography; COENZYME-A; STRUCTURE REFINEMENT; 3-DIMENSIONAL STRUCTURE; LIGAND-BINDING; METABOLISM; EXPRESSION; SOFTWARE; RESIDUES; ACBP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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