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Investigation of in...
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Bergman, DanielSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden
(författare)
Investigation of interference from canine anti-mouse antibodies in hormone immunoassays
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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2019-07-18
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Wiley,2019
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-389639
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-389639URI
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https://doi.org/10.1111/vcp.12764DOI
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https://res.slu.se/id/publ/100741URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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BACKGROUND: Canine anti-mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti-Müllerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.OBJECTIVES: We aimed to evaluate if canine anti-mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.METHODS: Twenty-nine positive and 25 negative samples for anti-mouse antibodies were analyzed for AMH, canine total thyroxine (TT4), canine thyroid-stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra-assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti-mouse antibodies and no visible gonads present were considered evidence of interference.RESULTS: Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti-mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%).CONCLUSIONS: Canine anti-mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Larsson, AndersUppsala universitet,Klinisk kemi(Swepub:uu)andlarss
(författare)
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Hamlin, HeleneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)50428
(författare)
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Ström Holst, BodilSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)47321
(författare)
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Sveriges lantbruksuniversitetInstitutionen för kliniska vetenskaper (KV)
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Sammanhörande titlar
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Ingår i:Veterinary clinical pathology: Wiley48:S1, s. 59-690275-63821939-165X
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