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Adduct formation in...
Adduct formation in electrospray ionisation-mass spectrometry with hydrophilic interaction liquid chromatography is strongly affected by the inorganic ion concentration of the samples
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- Erngren, Ida (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Analytisk farmaceutisk kemi
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- Haglöf, Jakob (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Analytisk farmaceutisk kemi
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- Engskog, Mikael K R (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Analytisk farmaceutisk kemi
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- Nestor, Marika, 1976- (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap
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- Hedeland, Mikael (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Natl Vet Inst SVA, Dept Chem Environm & Feed Hyg, Uppsala, Sweden,Analytisk farmaceutisk kemi
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- Arvidsson, Torbjörn (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Med Prod Agcy, Uppsala, Sweden,Analytisk farmaceutisk kemi
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- Pettersson, Curt (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Analytisk farmaceutisk kemi
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(creator_code:org_t)
- ELSEVIER SCIENCE BV, 2019
- 2019
- Engelska.
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Ingår i: Journal of Chromatography A. - : ELSEVIER SCIENCE BV. - 0021-9673 .- 1873-3778. ; 1600, s. 174-182
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Hydrophilic interaction liquid chromatography (HILIC)/electrospray ionisation-mass spectrometry (ESI-MS) has gained interest for the analysis of polar analytes in bioanalytical applications in recent years. However, ESI-MS is prone to adduct formation of analytes. In contrast to reversed phase chromatography, small inorganic ions have retention in HILIC, i.e. analytes and inorganic ions may co-elute, which could influence the adduct formation. In the present paper, it was demonstrated that the co-elution of sodium ions or potassium ions and analytes in HILIC/ESI-MS affect the adduct formation and that different concentrations of sodium ions and potassium ions in biological samples could have an impact on the quantitative response of the respective adducts as well as the quantitative response of the protonated adduct. The co-elution also lead to cluster formation of analytes and sodium formate or potassium formate, causing extremely complicated spectra. In analytical applications using HILIC/ESI-MS where internal standards are rarely used or not properly matched, great care needs to be taken to ensure minimal variation of inorganic ion concentration between samples. Moreover, the use of alkali metal ion adducts as quantitative target ions in relative quantitative applications should be made with caution if proper internal standards are not used.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Adduct formation
- Hydrophilic interaction liquid chromatography
- Mass spectrometry
- Screening
- Metabolomics
- Cluster formation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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