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An acoustofluidic p...
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Fornell, AnnaUppsala University,Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Embla
(författare)
An acoustofluidic platform for non-contact trapping of cell-laden hydrogel droplets compatible with optical microscopy
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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AIP Publishing,2019
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-392104
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-392104URI
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https://doi.org/10.1063/1.5108583DOI
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https://lup.lub.lu.se/record/430a4536-5414-4e3f-914a-fbfb95ea80aeURI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Production of cell-laden hydrogel droplets as miniaturized niches for 3D cell culture provides a new route for cell-based assays. Such production can be enabled by droplet microfluidics and here we present a droplet trapping system based on bulk acoustic waves for handling hydrogel droplets in a continuous flow format. The droplet trapping system consists of a glass capillary equipped with a small piezoelectric transducer. By applying ultrasound (4 MHz), a localized acoustic standing wave field is generated in the capillary, trapping the droplets in a well-defined cluster above the transducer area. The results show that the droplet cluster can be retained at flow rates of up to 76 mu l/min, corresponding to an average flow speed of 3.2 mm/s. The system allows for important operations such as continuous perfusion and/or addition of chemical reagents to the encapsulated cells with in situ optical access. This feature is demonstrated by performing on-chip staining of the cell nuclei. The key advantages of this trapping method are that it is label-free and gentle and thus well-suited for biological applications. Moreover, the droplets can easily be released on-demand, which facilitates downstream analysis. It is envisioned that the presented droplet trapping system will be a valuable tool for a wide range of multistep assays as well as long-term monitoring of cells encapsulated in gel-based droplets.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Johannesson, CarlLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)bme-caj
(författare)
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Searle, Sean S.,1991-Uppsala University,Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Natl Univ Singapore, Dept Biomed Engn, Fac Engn,,Embla,National University of Singapore(Swepub:uu)sease980
(författare)
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Happstadius, AxelLund University
(författare)
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Nilsson, JohanLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-jni
(författare)
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Tenje, MariaUppsala University,Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Embla(Swepub:lu)ftf-mit
(författare)
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Uppsala universitetMikrosystemteknik
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biomicrofluidics: AIP Publishing131932-1058
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