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Chrna2-OLM interneu...
Chrna2-OLM interneurons display different membrane properties and h-current magnitude depending on dorsoventral location
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- Hilscher, Markus M (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Univ Fed Rio Grande do Norte, Inst Brain, Ave Nascimento de Castro 2155, BR-59056045 Natal, RN, Brazil;Vienna Univ Technol, Inst Anal & Sci Comp, Vienna, Austria
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- Nogueira, Ingrid (författare)
- Univ Fed Rio Grande do Norte, Inst Brain, Ave Nascimento de Castro 2155, BR-59056045 Natal, RN, Brazil
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- Mikulovic, Sanja, 1986- (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi
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- Kullander, Klas, 1966- (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi
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- Leão, Richardson Naves (författare)
- Uppsala universitet,Genetisk utvecklingsbiologi,Univ Fed Rio Grande do Norte, Inst Brain, Ave Nascimento de Castro 2155, BR-59056045 Natal, RN, Brazil
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- Leão, Katarina E. (författare)
- Univ Fed Rio Grande do Norte, Inst Brain, Ave Nascimento de Castro 2155, BR-59056045 Natal, RN, Brazil
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(creator_code:org_t)
- 2019-07-13
- 2019
- Engelska.
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Ingår i: Hippocampus. - : Wiley. - 1050-9631 .- 1098-1063. ; 29:12, s. 1224-1237
- Relaterad länk:
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https://repositorio....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The hippocampus is an extended structure displaying heterogeneous anatomical cell layers along its dorsoventral axis. It is known that dorsal and ventral regions show different integrity when it comes to functionality, innervation, gene expression, and pyramidal cell properties. Still, whether hippocampal interneurons exhibit different properties along the dorsoventral axis is not known. Here, we report electrophysiological properties of dorsal and ventral oriens lacunosum moleculare (OLM) cells from coronal sections of the Chrna2-cre mouse line. We found dorsal OLM cells to exhibit a significantly more depolarized resting membrane potential compared to ventral OLM cells, while action potential properties were similar between the two groups. We found ventral OLM cells to show a higher initial firing frequency in response to depolarizing current injections but also to exhibit a higher spike-frequency adaptation than dorsal OLM cells. Additionally, dorsal OLM cells displayed large membrane sags in response to negative current injections correlating with our results showing that dorsal OLM cells have more hyperpolarization-activated current (I-h) compared to ventral OLM cells. Immunohistochemical examination indicates the h-current to correspond to hyperpolarization-activated cyclic nucleotide-gated subunit 2 (HCN2) channels. Computational studies suggest that I-h in OLM cells is essential for theta oscillations in hippocampal circuits, and here we found dorsal OLM cells to present a higher membrane resonance frequency than ventral OLM cells. Thus, our results highlight regional differences in membrane properties between dorsal and ventral OLM cells allowing this interneuron to differently participate in the generation of hippocampal theta rhythms depending on spatial location along the dorsoventral axis of the hippocampus.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Chrna2-cre
- dorsoventral
- HCN
- H-resonance
- hyperpolarization-activated current
- OLM cell
- septotemporal
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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