SwePub
Sök i LIBRIS databas

  Utökad sökning

onr:"swepub:oai:DiVA.org:uu-418964"
 

Sökning: onr:"swepub:oai:DiVA.org:uu-418964" > iTRAQ-Based Proteom...

  • Alejandro Zamora-Briseno, JesusInst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico. (författare)

iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected WithPanulirus argusVirus 1 : Metabolic and Physiological Implications

  • Artikel/kapitelEngelska2020

Förlag, utgivningsår, omfång ...

  • 2020-05-29
  • Frontiers Media SA,2020
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:uu-418964
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-418964URI
  • https://doi.org/10.3389/fmicb.2020.01084DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The Caribbean spiny lobsterPanulirus argus(Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virusPanulirus argusVirus 1 (PaV1) that causes a systemic and chronic-degenerative infection in juvenile spiny lobstersP. argus. To date, there is no available information regarding the host alterations induced by this pathogen at the molecular level. In the present study, comparative proteomic analyses of the changes in the hepatopancreas between infected and non-infected juvenile lobsters were analyzed by isobaric tags for relative and absolute quantitation (iTRAQ) coupled to synchronous precursor selection (SPS)-based MS3. We identified a total of 636 proteins, being 68 down-regulated and 71 up-regulated proteins. Among the down-regulated proteins, we identified several enzymes involved in the metabolism of hormones and lipids, digestive proteases and glycosidases, while proteins associated with the histone core, protein synthesis, immune response and RNA regulation were up-regulated. Several misregulated enzymes involved in the regulation of neuromodulators were also identified. RT-qPCR assays were used to validate the expression of transcripts encoding for selected differential proteins that were in concordance to proteomic data, as well as the tendency observed in the enzymatic activities of trypsin, chymotrypsin, and glycosidase. In a similar way, we observed glycogen reduction in muscle, and an increase in plasma acylglycerides and glucose, which may be explained by proteomic data. This study provides the first insight into the molecular changes in the hepatopancreas of Caribbean spiny lobsters associated to PaV1 infection. Data provided herein would help to clarify the origin of the molecular misregulations observed at macroscopic level in this host-pathogen interaction.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Ruiz-May, ElielCluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico. (författare)
  • Miguel Elizalde-Contreras, JoseCluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico. (författare)
  • Margarita Hernandez-Velazquez, IoreniInst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico. (författare)
  • Hernandez-Perez, AriadneUppsala universitet,Jämförande fysiologi(Swepub:uu)arihe244 (författare)
  • Guadalupe Fuentes-Garcia, AnaInst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico. (författare)
  • Herrera-Salvatierra, NancyInst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico. (författare)
  • Briones-Fourzan, PatriciaUniv Nacl Autonoma Mexico, Isnt Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Puerto Morelos, Mexico. (författare)
  • Pascual-Jimenez, CristinaUniv Nacl Autonoma Mexico, Unidad Multidisciplinaria Docencia & Invest, Fac Ciencias, Sisal, Mexico. (författare)
  • Lozano-Alvarez, EnriqueUniv Nacl Autonoma Mexico, Isnt Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Puerto Morelos, Mexico. (författare)
  • Rodriguez-Canul, RossannaInst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico. (författare)
  • Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico. (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Frontiers in Microbiology: Frontiers Media SA111664-302X

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy