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Selective translati...
Selective translation by alternative bacterial ribosomes
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- Chen, Yu-Xiang (författare)
- Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine, 100084 Beijing, China; Division of Experimental Medicine, University of California, San Francisco, CA 94110
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- Xu, Zhi-Yu (författare)
- Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine, 100084 Beijing, China; Ministry of Education Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China
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- Ge, Xueliang (författare)
- Uppsala universitet,Molekylärbiologi
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- Hong, Jia-Yao (författare)
- Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine, 100084 Beijing, China
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- Sanyal, Suparna (författare)
- Uppsala universitet,Molekylärbiologi
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- Lu, Zhi John (författare)
- Tsinghua Univ, Sch Life Sci, Ctr Synthet & Syst Biol, Minist Educ,Key Lab Bioinformat, Beijing 100084, Peoples R China
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- Javid, Babak (författare)
- Centre for Global Health and Infectious Diseases, Collaborative Innovation Centre for the Diagnosis and Treatment of Infectious Diseases, Tsinghua University School of Medicine, 100084 Beijing, China; Division of Experimental Medicine, University of California, San Francisco, CA 94110; Beijing Advanced Innovation Center in Structural Biology, 100084 Beijing, China
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(creator_code:org_t)
- 2020-07-28
- 2020
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 117:32, s. 19487-19496
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://www.pnas.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Alternative ribosome subunit proteins are prevalent in the genomes of diverse bacterial species, but their functional significance is controversial. Attempts to study microbial ribosomal heterogeneity have mostly relied on comparing wild-type strains with mutants in which subunits have been deleted, but this approach does not allow direct comparison of alternate ribosome isoforms isolated from identical cellular contexts. Here, by simultaneously purifying canonical and alternative RpsR ribosomes from Mycobacterium smegmatis, we show that alternative ribosomes have distinct translational features compared with their canonical counterparts. Both alternative and canonical ribosomes actively take part in protein synthesis, although they translate a subset of genes with differential efficiency as measured by ribosome profiling. We also show that alternative ribosomes have a relative defect in initiation complex formation. Furthermore, a strain of M. smegmatis in which the alternative ribosome protein operon is deleted grows poorly in iron-depleted medium, uncovering a role for alternative ribosomes in iron homeostasis. Our work confirms the distinct and nonredundant contribution of alternative bacterial ribosomes for adaptation to hostile environments.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- mycobacterium
- alternative ribosomes
- ribosome profiling
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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