Sökning: onr:"swepub:oai:DiVA.org:uu-419799" > zOPT: an open sourc...
Fältnamn | Indikatorer | Metadata |
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000 | 03407naa a2200361 4500 | |
001 | oai:DiVA.org:uu-419799 | |
003 | SwePub | |
008 | 200916s2020 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4197992 URI |
024 | 7 | a https://doi.org/10.1364/BOE.3935192 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Zhang, Hanqingu Uppsala universitet,Bildanalys och människa-datorinteraktion,Science for Life Laboratory, SciLifeLab,Avdelningen för visuell information och interaktion4 aut0 (Swepub:uu)hanzh404 |
245 | 1 0 | a zOPT: an open source optical projection tomography system and methods for rapid 3D zebrafish imaging |
264 | 1 | b The Optical Society,c 2020 |
338 | a electronic2 rdacarrier | |
520 | a Optical projection tomography (OPT) is a 3D imaging alternative to conventional microscopy which allows imaging of millimeter-sized object with isotropic micrometer resolution. The zebrafish is an established model organism and an important tool used in genetic and chemical screening. The size and optical transparency of the embryo and larva makes them well suited for imaging using OPT. Here, we present an open-source implementation of an OPT platform, built around a customized sample stage, 3D-printed parts and open source algorithms optimized for the system. We developed a versatile automated workflow including a two-step image processing approach for correcting the center of rotation and generating accurate 3D reconstructions. Our results demonstrate high-quality 3D reconstruction using synthetic data as well as real data of live and fixed zebrafish. The presented 3D-printable OPT platform represents a fully open design, low-cost and rapid loading and unloading of samples. Our system offers the opportunity for researchers with different backgrounds to setup and run OPT for large scale experiments, particularly in studies using zebrafish larvae as their key model organism. | |
650 | 7 | a TEKNIK OCH TEKNOLOGIERx Medicinteknikx Medicinsk bildbehandling0 (SwePub)206032 hsv//swe |
650 | 7 | a ENGINEERING AND TECHNOLOGYx Medical Engineeringx Medical Image Processing0 (SwePub)206032 hsv//eng |
700 | 1 | a Waldmann, Laurau Uppsala universitet,Evolution och utvecklingsbiologi4 aut0 (Swepub:uu)lauwa544 |
700 | 1 | a Manuel, Remyu Uppsala universitet,Boije: Zebrafiskens neuronala nätverk4 aut0 (Swepub:uu)remma294 |
700 | 1 | a Boije, Henriku Uppsala universitet,Boije: Zebrafiskens neuronala nätverk4 aut0 (Swepub:uu)henbo576 |
700 | 1 | a Haitina, Tatjana,c Docent,d 1981-u Uppsala universitet,Evolution och utvecklingsbiologi4 aut0 (Swepub:uu)tahai516 |
700 | 1 | a Allalou, Amin,d 1981-u Uppsala universitet,Science for Life Laboratory, SciLifeLab,Bildanalys och människa-datorinteraktion4 aut0 (Swepub:uu)amial914 |
710 | 2 | a Uppsala universitetb Bildanalys och människa-datorinteraktion4 org |
773 | 0 | t Biomedical Optics Expressd : The Optical Societyg 11:8, s. 4290-4305q 11:8<4290-4305x 2156-7085 |
856 | 4 | u https://doi.org/10.1364/BOE.393519y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1467761/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-419799 |
856 | 4 8 | u https://doi.org/10.1364/BOE.393519 |
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