Sökning: onr:"swepub:oai:DiVA.org:uu-420203" >
Bioactive glass S53...
Bioactive glass S53P4 eradicatesStaphylococcus aureusin biofilm/planktonic statesin vitro
-
- Gronseth, Torstein (författare)
- Univ Oslo, Inst Clin Med, Oslo, Norway.;Oslo Univ Hosp, Dept Otolaryngol Head & Neck Surg, Oslo, Norway.
-
- Vestby, Lene K. (författare)
- Norwegian Vet Inst, Dept Anal & Diagnost, Sect Immunol & Virol, Oslo, Norway.
-
- Nesse, Live L. (författare)
- Norwegian Vet Inst, Dept Anim Hlth & Food Safety, Oslo, Norway.
-
visa fler...
-
- von Unge, Magnus (författare)
- Uppsala universitet,Centrum för klinisk forskning, Västerås,Akershus Univ Hosp, Dept Otolaryngol Head & Neck Surg, Akershus, Norway.;Akershus Univ Hosp, Dept Otolaryngol Head & Neck Surg, Oslo, Norway
-
- Silvola, Juha T. (författare)
- Univ Oslo, Inst Clin Med, Oslo, Norway.;Oslo Univ Hosp, Dept Otolaryngol Head & Neck Surg, Oslo, Norway.;Akershus Univ Hosp, Dept Otolaryngol Head & Neck Surg, Akershus, Norway.;Akershus Univ Hosp, Dept Otolaryngol Head & Neck Surg, Oslo, Norway.
-
visa färre...
-
Univ Oslo, Inst Clin Med, Oslo, Norway;Oslo Univ Hosp, Dept Otolaryngol Head & Neck Surg, Oslo, Norway. Norwegian Vet Inst, Dept Anal & Diagnost, Sect Immunol & Virol, Oslo, Norway. (creator_code:org_t)
- 2020-06-18
- 2020
- Engelska.
-
Ingår i: Upsala Journal of Medical Sciences. - : TAYLOR & FRANCIS LTD. - 0300-9734 .- 2000-1967. ; 125:3, s. 217-225
- Relaterad länk:
-
https://uu.diva-port... (primary) (Raw object)
-
visa fler...
-
https://ujms.net/ind...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background:Increasing antimicrobial resistance to antibiotics is a substantial health threat. Bioactive glass S53P4 (BAG) has an antimicrobial effect that can reduce the use of antibiotics. The aim of this study was to evaluate the antimicrobial efficacy of BAGin vitroon staphylococci in biofilm and in planktonic form. Secondary aims were to investigate whether supernatant fluid primed from BAG retains the antibacterial capacity and if ciprofloxacin enhances the effect. Methods:BAG-S53P4 granules, <45 mu m, primed in tryptic soy broth (TSB) were investigated with granules present in TSB (100 mg/mL) and after removal of granules (100, 200, and 400 mg/mL). The efficacy of BAG to eradicateStaphylococcus aureusbiofilmin vitrowas tested using 10 different clinical strains and 1 reference strain in three test systems: the biofilm-oriented antiseptic test based on metabolic activity, the biofilm bactericidal test based on culturing surviving bacteria, and confocal laser scanning microscopy (CLSM) combined with LIVE/DEAD staining. Results:Exposure to 48 h primed BAG granules (100 mg/mL) produced bactericidal effects in 11/11 strains (p = 0.001), and CLSM showed reduction of viable bacteria in biofilm (p = 0.001). Supernatant primed 14 days, 400 mg/mL, reduced metabolic activity (p < 0.001), showed bactericidal effects for 11/11 strains (p = 0.001), and CLSM showed fewer viable bacteria (p = 0.001). The supernatant primed for 48 h, or in concentrations lower than 400 mg/mL at 14 days, did not completely eradicate biofilm. Conclusion:Direct exposure to BAG granules, or primed supernatant fluid, effectively eradicatedS. aureusin biofilm. The anti-biofilm effect is time- and concentration-dependent. When BAG had reached its full antimicrobial effect, ciprofloxacin had no additional effect.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- BAG
- biofilm
- confocal laser scanning microscopy
- Staphylococcus aureus
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas