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Altered IL-32 Signa...
Altered IL-32 Signaling in Abdominal Aortic Aneurysm
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- Bengts, Sophy (författare)
- Linköpings universitet,Avdelningen för läkemedelsforskning,Medicinska fakulteten
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- Shamoun, Levar (författare)
- Jönköping Cty, Dept Lab Med, Div Med Diagnost, Jönköping, Sweden.;Uppsala Univ, Dept Med Cell Biol, BOX 571, SE-75123 Uppsala, Sweden.
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- Kunath, Anne (författare)
- Uppsala universitet,Institutionen för medicinsk cellbiologi,Uppsala Univ, Sweden
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- Appelgren, Daniel (författare)
- Linköpings universitet,Avdelningen för diagnostik och specialistmedicin,Medicinska fakulteten
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- Welander, Martin (författare)
- Linköpings universitet,Avdelningen för diagnostik och specialistmedicin,Medicinska fakulteten,Region Östergötland, Thorax-kärlkliniken i Östergötland
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- Björck, Martin (författare)
- Uppsala universitet,Kärlkirurgi,Uppsala Univ, Sweden
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- Wanhainen, Anders (författare)
- Uppsala universitet,Kärlkirurgi,Uppsala Univ, Sweden
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- Wågsäter, Dick, Professor (författare)
- Linköpings universitet,Uppsala universitet,Institutionen för medicinsk cellbiologi,Linköping Univ, Dept Med & Hlth Sci, Div Drug Res, Linköping, Sweden.,Avdelningen för läkemedelsforskning,Medicinska fakulteten,Uppsala Univ, Sweden
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(creator_code:org_t)
- 2020-05-20
- 2020
- Engelska.
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Ingår i: Journal of Vascular Research. - : KARGER. - 1018-1172 .- 1423-0135. ; 57:4, s. 236-244
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Abstract
Ämnesord
Stäng
- Introduction and Objective:Interleukin (IL)-32 is a pro-inflammatory cytokine not previously studied in relation to abdominal aortic aneurysm (AAA). The aim of this study was to elucidate the expression and localization of IL-32 in AAA.Methods:Expression and localization of IL-32 in human aortic tissue was studied with immunohistochemical analysis and Western blot (AAA:n= 5; controls:n= 4). ELISA was used to measure IL-32 in human plasma samples (AAA:n= 140; controls:n= 37) and in media from cultured peripheral blood mononuclear cells (PBMCs) from 3 healthy donors. IL-32 mRNA in PBMCs, endothelial cells, aortic smooth muscle cells (SMCs), and aortic tissue samples of AAA (n= 16) and control aortas (n= 9) was measured with qPCR.Results:IL-32 was predominantly expressed in SMCs and T-cell-rich areas. Highest mRNA expression was observed in the intima/media layer of the AAA. A weaker protein expression was detected in non-aneurysmal aortas. Expression of IL-32 was confirmed in isolated T cells, macrophages, endothelial cells, and SMCs, where expression was also inducible by cytokines such as interferon-gamma. There was no difference in IL-32 expression in plasma between patients and controls.Conclusion:IL-32 signaling is altered locally in AAA and could potentially play an important role in aneurysm development. Further studies using animal models would be helpful to study its potential role in AAA disease.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Nyckelord
- Abdominal aortic aneurysm
- Inflammation
- Leukocytes
- T cells
- Cytokines
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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