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A real-time cell-bi...
A real-time cell-binding assay reveals dynamic features of STxB-Gb3 cointernalization and STxB-mediated cargo delivery into cancer cells
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- Encarnacao, Joao Crispim, Master, 1990- (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap,Ridgeview Instruments AB, Dag Hammarskjolds Vag 36A,Sci Pk, S-75237 Uppsala, Sweden.
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- Napolitano, Valeria (författare)
- Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, Poland.;Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Krakow, Poland.
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- Opassi, Giulia, 1988- (författare)
- Uppsala universitet,Institutionen för kemi - BMC
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- Danielson, U. Helena, Professor, 1959- (författare)
- Uppsala universitet,Institutionen för kemi - BMC
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- Dubin, Grzegorz (författare)
- Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, Poland.
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- Popowicz, Grzegorz M. (författare)
- Helmholtz Zentrum Munchen, Inst Struct Biol, Neuherberg, Germany.;Tech Univ Munich, Ctr Integrated Prot Sci Munich, Dept Chem, Chair Biomol NMR, Garching, Germany.
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- Munier-Lehmann, Helene (författare)
- Inst Pasteur, Dept Biol Struct & Chim, Unite Chim & Biocatalyse, CNRS,UMR3523, Paris, France.
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- Buijs, Jos (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap,Ridgeview Instruments AB, Dag Hammarskjolds Vag 36A,Sci Pk, S-75237 Uppsala, Sweden.
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- Andersson, Karl, 1972- (författare)
- Uppsala universitet,Medicinsk strålningsvetenskap,Ridgeview Instruments AB, Dag Hammarskjolds Vag 36A,Sci Pk, S-75237 Uppsala, Sweden.
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- Bjorkelund, Hanna (författare)
- Ridgeview Instruments AB, Dag Hammarskjolds Vag 36A,Sci Pk, S-75237 Uppsala, Sweden.
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(creator_code:org_t)
- 2020-06-23
- 2020
- Engelska.
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Ingår i: FEBS Letters. - : WILEY. - 0014-5793 .- 1873-3468. ; 594:15, s. 2406-2420
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The interaction between the Shiga toxin B-subunit (STxB) and its globotriaosylceramide receptor (Gb3) has a high potential for being exploited for targeted cancer therapy. The primary goal of this study was to evaluate the capacity of STxB to carry small molecules and proteins as cargo into cells. For this purpose, an assay was designed to provide real-time information about the StxB-Gb3 interaction as well as the dynamics and mechanism of the internalization process. The assay revealed the ability to distinguish the process of binding to the cell surface from internalization and presented the importance of receptor and STxB clustering for internalization. The overall setup demonstrated that the binding mechanism is complex, and the concept of affinity is difficult to apply. Hence, time-resolved methods, providing detailed information about the interaction of STxB with cells, are critical for the optimization of intracellular delivery.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- binding kinetics
- cancer
- cell surface receptor
- real-time cell-binding assays
- receptor internalization
- Shiga toxin
Publikations- och innehållstyp
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Encarnacao, Joao ...
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Napolitano, Vale ...
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Opassi, Giulia, ...
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Danielson, U. He ...
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Dubin, Grzegorz
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Popowicz, Grzego ...
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Munier-Lehmann, ...
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Buijs, Jos
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Andersson, Karl, ...
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Bjorkelund, Hann ...
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FEBS Letters
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