Sökning: onr:"swepub:oai:DiVA.org:uu-428936" >
Isopedopeptins A-H :
Isopedopeptins A-H : Cationic Cyclic Lipodepsipeptides from Pedobacter cryoconitis UP508 Targeting WHO Top-Priority Carbapenem-Resistant Bacteria
-
- Nord, Christina (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, Uppsala BioCtr, Dept Mol Sci, SE-75007 Uppsala, Sweden.
-
- Bjerketorp, Joakim (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Ultupharma,Swedish Univ Agr Sci, Uppsala BioCtr, Dept Mol Sci, SE-75007 Uppsala, Sweden.;Ultupharma AB, SE-75236 Uppsala, Sweden.
-
- Levenfors, Jolanta (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Ultupharma,Swedish Univ Agr Sci, Uppsala BioCtr, Dept Mol Sci, SE-75007 Uppsala, Sweden.;Ultupharma AB, SE-75236 Uppsala, Sweden.
-
visa fler...
-
- Cao, Sha (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Strömstedt, Adam A., Docent, 1977- (författare)
- Uppsala universitet,Farmakognosi
-
- Guss, Bengt (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, SE-75007 Uppsala, Sweden.
-
- Larsson, Rolf (författare)
- Uppsala universitet,Cancerfarmakologi och beräkningsmedicin
-
- Hughes, Diarmaid, 1956- (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Öberg, Bo (författare)
- Uppsala universitet,Institutionen för läkemedelskemi,Ultupharma AB, SE-75236 Uppsala, Sweden.
-
- Broberg, Anders (författare)
- Swedish Univ Agr Sci, Uppsala BioCtr, Dept Mol Sci, SE-75007 Uppsala, Sweden.
-
- Broberg, Henrik (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences
-
visa färre...
-
(creator_code:org_t)
-
- 2020-10-15
- 2020
- Engelska.
-
Ingår i: ACS Chemical Biology. - : AMER CHEMICAL SOC. - 1554-8929 .- 1554-8937. ; 15:11, s. 2937-2944
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://pubs.acs.org...
-
https://pub.epsilon.... (primary) (Raw object) (free)
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
https://res.slu.se/i...
-
visa färre...
Abstract
Ämnesord
Stäng
- Pedobacter cryoconitis strain UP508 was isolated from a soil sample using a mixture of ampicillin, kanamycin, and nalidixic acid for selection. UP508 was found to produce >30 unknown antibacterial peptides, of which eight, isopedopeptins A-H (1-8), were isolated by bioassay-guided fractionation and characterized with respect to structures and biological properties. Compounds 1-8 were all composed of nine amino acid residues and one 3-hydroxy fatty acid residue, and the structures were ring-closed via an ester bond from the C-terminal aspartic acid to the 3-hydroxy fatty acid. The differences between the peptides were the size and branching of the 3-hydroxy fatty acid and the presence of a valine or a 3-hydroxyvaline residue. The isopedopeptins mainly had activity against Gram-negative bacteria, and isopedopeptin B (2), which had the best combination of antibacterial activity, in vitro cytotoxicity, and hemolytic properties, was selected for further studies against a larger panel of Gram-negative bacteria. Isopedopeptin B was found to have good activity against strains of WHO top-priority Gram-negative bacteria, i.e., carbapenem-resistant Acinetobacter baumannii, Escherichia coli, and Pseudomonas aeruginosa, with minimal inhibitory concentrations (MIC) down to 1, 2, and 4 mu g/mL, respectively. Furthermore, compound 2 had activity against colistin-resistant strains of A. baumannii, E. coli, and Klebsiella pneumoniae, with a MIC down to 8, 2, and 4 mu g/mL, respectively. Compound 6 was tested in an E. coli liposome system where it induced significant leakage, indicating membrane disruption as one mechanism involved in isopedopeptin antibacterial activity. Isopedopeptin B stands out as a promising candidate for further studies with the goal to develop a new antibiotic drug.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Nord, Christina
-
Bjerketorp, Joak ...
-
Levenfors, Jolan ...
-
Cao, Sha
-
Strömstedt, Adam ...
-
Guss, Bengt
-
visa fler...
-
Larsson, Rolf
-
Hughes, Diarmaid ...
-
Öberg, Bo
-
Broberg, Anders
-
Broberg, Henrik
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Mikrobiologi
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
ACS Chemical Bio ...
- Av lärosätet
-
Uppsala universitet
-
Sveriges Lantbruksuniversitet