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Assessing Cell Viab...
Assessing Cell Viability Effects of Opioids in Primary Cortical Cells from Rat.
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- Grönbladh, Alfhild, 1983- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Nylander, Erik, 1986- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Zelleroth, Sofia, 1990- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Hallberg, Mathias, 1971- (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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(creator_code:org_t)
- 2020-09-26
- 2021
- Engelska.
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer Nature. - 1064-3745 .- 1940-6029. ; 2201, s. 171-180
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Although the number of studies that have examined the impact of opioids on cell viability is very limited, it has clearly shown that opioids commonly used in the clinic can both decrease neurogenesis and induce cell death. These negative effects induced by opioids are worrying and there is a need for further in-depth investigations addressing the impact of opioids on cell function and cell viability. A useful in vitro approach for studying the effects of opioids on cellular function and viability is using primary cortical cell cultures obtained from embryonic day 17 (E17) rat embryos. These cell cultures contain both neurons and glial cells that provide a more physiologically relevant culture condition when compared to the use of various commercially available cell lines. The primary cortical cells can be cultivated in 96-well plates, treated with various concentrations of opioids, and cell viability functions such as mitochondrial function and membrane integrity can easily be assessed using specific colorimetric assays.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- Cell viability
- Cortex
- In vitro
- LDH
- MTT
- Membrane integrity
- Mitochondrial function
- Primary cell culture
- Rat
- Pharmacology
- Farmakologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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