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Label-Free Optical ...
Label-Free Optical Detection of DNA Polymerase Docking on Solid-State Nanopore Arrays
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- Li, Shiyu (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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- Zeng, Shuangshuang (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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- Wen, Chenyu (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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visa fler...
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- Zhang, Zhen, 1979- (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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- Hjort, Klas, 1964- (författare)
- Uppsala universitet,Mikrosystemteknik
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- Zhang, Shi-Li (författare)
- Uppsala universitet,Fasta tillståndets elektronik
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Integration of motor enzymes with biological nanopores has enabled commercial DNA sequencing, yet studies of the similar principle applying to solid-state nanopores are limited. Here, we demonstrate the label-free optical detection of phi29 DNA polymerase (DNAP) docking onto truncated-pyramidal nanopores (TPP). Atomic layer deposition of hafnium oxide is employed to shrink the pore opening size of original silicon (Si) TPP to sub-10 nm. Ionic current measurements of single TPP of an opening size comparable to DNAP show that polymerase-DNA complexes can temporally dock onto the TPP with a certain docking orientation, while the majority are translocation events. A label-free optical detection method using Ca2+ sensitive dye is employed to detect the docking of DNAP on 5-by-5 nanopore arrays. The results of the proof-of-concept experiment show that this label-free detection strategy is capable of accessing the docking events of DNAP on solid-state nanopore arrays.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Elektroteknik och elektronik -- Annan elektroteknik och elektronik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Electrical Engineering, Electronic Engineering, Information Engineering -- Other Electrical Engineering, Electronic Engineering, Information Engineering (hsv//eng)
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)