Sökning: onr:"swepub:oai:DiVA.org:uu-435198" > Culturable bacteria...
Fältnamn | Indikatorer | Metadata |
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000 | 06347naa a2200661 4500 | |
001 | oai:DiVA.org:uu-435198 | |
003 | SwePub | |
008 | 210226s2021 | |||||||||||000 ||eng| | |
009 | oai:slubar.slu.se:109965 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4351982 URI |
024 | 7 | a https://doi.org/10.1186/s12936-020-03574-12 DOI |
024 | 7 | a https://res.slu.se/id/publ/1099652 URI |
040 | a (SwePub)uud (SwePub)slu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Rocha, Elerson Matosu Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut |
245 | 1 0 | a Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential |
264 | c 2021-01-13 | |
264 | 1 | b BioMed Central (BMC),c 2021 |
338 | a electronic2 rdacarrier | |
500 | a Correction in: Malaria Journal, vol. 20, issue 1, article number 83.DOI: 10.1186/s12936-021-03609-1 | |
520 | a Background: Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans.Methods: Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates.Results: A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation.Conclusion: Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature. | |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
650 | 7 | a NATURVETENSKAPx Biologix Ekologi0 (SwePub)106112 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Ecology0 (SwePub)106112 hsv//eng |
653 | a Mosquito | |
653 | a Malaria | |
653 | a Microbiota | |
653 | a Vector-borne disease | |
653 | a Amazon forest | |
700 | 1 | a Marinotti, Osvaldou MTEKPrime, Aliso Viejo, CA USA.4 aut |
700 | 1 | a Serrao, Deidre Machadou Univ Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Correa, Laura Vianau Univ Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Katak, Ricardo de Melou Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Juan, Campos-de-Oliveiru Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Muniz, Veranilce Alvesu Univ Estadual Amazonas MBT, UEA, Manaus, Amazonas, Brazil. Univ Estadual Amazonas BIONORTE, UEA, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a de Oliveira, Marta Rodrigues4 aut |
700 | 1 | a Ferreira, Joaquimu INPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Pessoa, Marcos Cezar Fernandesu Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Roque, Rosemary Aparecidau INPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a da Mota, Adolfo Joseu Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.4 aut |
700 | 1 | a Onorati, Pierou Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för ekologi,Department of Ecology,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, S-75007 Uppsala, Sweden.4 aut |
700 | 1 | a Souza-Neto, Jayme A.u Sao Paulo State Univ UNESP, Cent Multi User Lab, Dept Bioproc & Biotechnol, Sch Agr Sci, Botucatu, SP, Brazil.4 aut |
700 | 1 | a Terenius, Olleu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Mikrobiologi,Swedish Univ Agr Sci SLU, Dept Ecol, Box 7044, S-75007 Uppsala, Sweden.,Institutionen för ekologi,Department of Ecology,Uppsala University4 aut0 (Swepub:slu)47992 |
700 | 1 | a Tadei, Wanderli Pedrou INPA, Inst Nacl Pesquisas Amazonia, Lab Malaria & Dengue, Manaus, Amazonas, Brazil.4 aut |
710 | 2 | a Univ Fed Amazonas PPGBIOTEC UFAM, Programa Posgrad Biotecnol, Manaus, Amazonas, Brazil.b MTEKPrime, Aliso Viejo, CA USA.4 org |
710 | 2 | a Sveriges lantbruksuniversitet |
773 | 0 | t Malaria Journald : BioMed Central (BMC)g 20q 20x 1475-2875 |
856 | 4 | u https://doi.org/10.1186/s12936-020-03574-1y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1531419/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://malariajournal.biomedcentral.com/track/pdf/10.1186/s12936-020-03574-1 |
856 | 4 | u https://pub.epsilon.slu.se/id/eprint/23270/contentsx primaryx Raw objectx freey FULLTEXT |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-435198 |
856 | 4 8 | u https://doi.org/10.1186/s12936-020-03574-1 |
856 | 4 8 | u https://res.slu.se/id/publ/109965 |
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