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Double Mutant Cycle...
Double Mutant Cycles as a Tool to Address Folding, Binding, and Allostery
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- Pagano, Livia (författare)
- Sapienza Univ Roma, Ist Pasteur, Fdn Cenci Bolognetti, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy.
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- Toto, Angelo (författare)
- Sapienza Univ Roma, Ist Pasteur, Fdn Cenci Bolognetti, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy.
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- Malagrino, Francesca (författare)
- Sapienza Univ Roma, Ist Pasteur, Fdn Cenci Bolognetti, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy.
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- Visconti, Lorenzo (författare)
- Sapienza Univ Roma, Ist Pasteur, Fdn Cenci Bolognetti, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy.
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- Jemth, Per (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Gianni, Stefano (författare)
- Sapienza Univ Roma, Ist Pasteur, Fdn Cenci Bolognetti, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy.
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Sapienza Univ Roma, Ist Pasteur, Fdn Cenci Bolognetti, Dipartimento Sci Biochim A Rossi Fanelli, I-00185 Rome, Italy Institutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)
- 2021-01-15
- 2021
- Engelska.
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Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 22:2
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Quantitative measurement of intramolecular and intermolecular interactions in protein structure is an elusive task, not easy to address experimentally. The phenomenon denoted 'energetic coupling' describes short- and long-range interactions between two residues in a protein system. A powerful method to identify and quantitatively characterize long-range interactions and allosteric networks in proteins or protein-ligand complexes is called double-mutant cycles analysis. In this review we describe the thermodynamic principles and basic equations that underlie the double mutant cycle methodology, its fields of application and latest employments, and caveats and pitfalls that the experimentalists must consider. In particular, we show how double mutant cycles can be a powerful tool to investigate allosteric mechanisms in protein binding reactions as well as elusive states in protein folding pathways.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- coupling energy
- site-directed mutagenesis
- interaction networks
Publikations- och innehållstyp
- ref (ämneskategori)
- for (ämneskategori)
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