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Proteomic compariso...
Proteomic comparison between different tissue preservation methods for identification of promising biomarkers of urothelial bladder cancer
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- Valdés, Alberto (författare)
- Uppsala universitet,Analytisk kemi,CSIC, Lab Food, Inst Food Sci Res, CIAL, Nicolas Cabrera 9, Madrid 28049, Spain
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- Bitzios, Athanasios (författare)
- Uppsala universitet,Analytisk kemi
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- Kassa, Eszter (författare)
- Uppsala universitet,Analytisk kemi
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- Shevchenko, Ganna (författare)
- Uppsala universitet,Analytisk kemi
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- Falk, Alexander, Research engineer, 1986- (författare)
- Uppsala universitet,Analytisk kemi
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- Malmström, Per-Uno (författare)
- Uppsala universitet,Urologkirurgi
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- Dragomir, Anca (författare)
- Uppsala universitet,Klinisk och experimentell patologi
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- Segersten, Ulrika (författare)
- Uppsala universitet,Urologkirurgi
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- Bergström Lind, Sara, 1977- (författare)
- Uppsala universitet,Analytisk kemi
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(creator_code:org_t)
- 2021-04-07
- 2021
- Engelska.
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 11:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Samples in biobanks are generally preserved by formalin-fixation and paraffin-embedding (FFPE) and/or optimal cutting temperature compound (OCT)-embedding and subsequently frozen. Mass spectrometry (MS)-based analysis of these samples is now available via developed protocols, however, the differences in results with respect to preservation methods needs further investigation. Here we use bladder urothelial carcinoma tissue of two different tumor stages (Ta/T1-non-muscle invasive bladder cancer (NMIBC), and T2/T3-muscle invasive bladder cancer (MIBC)) which, upon sampling, were divided and preserved by FFPE and OCT. Samples were parallel processed from the two methods and proteins were analyzed with label-free quantitative MS. Over 700 and 1200 proteins were quantified in FFPE and OCT samples, respectively. Multivariate analysis indicates that the preservation method is the main source of variation, but also tumors of different stages could be differentiated. Proteins involved in mitochondrial function were overrepresented in OCT data but missing in the FFPE data, indicating that these proteins are not well preserved by FFPE. Concordant results for proteins such as HMGCS2 (uniquely quantified in Ta/T1 tumors), and LGALS1, ANXA5 and plastin (upregulated in T2/T3 tumors) were observed in both FFPE and OCT data, which supports the use of MS technology for biobank samples and encourages the further evaluation of these proteins as biomarkers.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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- Av författaren/redakt...
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Valdés, Alberto
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Bitzios, Athanas ...
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Kassa, Eszter
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Shevchenko, Gann ...
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Falk, Alexander, ...
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Malmström, Per-U ...
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visa fler...
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Dragomir, Anca
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Segersten, Ulrik ...
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Bergström Lind, ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
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Scientific Repor ...
- Av lärosätet
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Uppsala universitet