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A droplet acoustofl...
A droplet acoustofluidic platform for time-controlled microbead-based reactions
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- Liu, Zhenhua, 1992- (författare)
- Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,EMBLA
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- Fornell, Anna (författare)
- Uppsala University,Lund University,Lunds universitet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,Lund Univ, Max IV Lab, SE-22100 Lund, Sweden,EMBLA,MAX IV-laboratoriet,MAX IV Laboratory
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- Tenje, Maria (författare)
- Uppsala University,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,EMBLA
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(creator_code:org_t)
- American Institute of Physics (AIP), 2021
- 2021
- Engelska.
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Ingår i: Biomicrofluidics. - : American Institute of Physics (AIP). - 1932-1058. ; 15:3
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Droplet microfluidics is a powerful method used to characterize chemical reactions at high throughput. Often detection is performed via in-line optical readout, which puts high demands on the detection system or makes detection of low concentration substrates challenging. Here, we have developed a droplet acoustofluidic chip for time-controlled reactions that can be combined with off-line optical readout. The principle of the platform is demonstrated by the enzymatic conversion of fluorescein diphosphate to fluorescein by alkaline phosphatase. The novelty of this work is that the time of the enzymatic reaction is controlled by physically removing the enzymes from the droplets instead of using chemical inhibitors. This is advantageous as inhibitors could potentially interact with the readout. Droplets containing substrate were generated on the chip, and enzyme-coupled microbeads were added into the droplets via pico-injection. The reaction starts as soon as the enzyme/bead complexes are added, and the reaction is stopped when the microbeads are removed from the droplets at a channel bifurcation. The encapsulated microbeads were focused in the droplets by acoustophoresis during the split, leaving the product in the side daughter droplet to be collected for the analysis (without beads). The time of the reaction was controlled by using different outlets, positioned at different lengths from the pico-injector. The enzymatic conversion could be measured with fluorescence readout in a separate PDMS based assay chip. We show the ability to perform time-controlled enzymatic assays in droplet microfluidics coupled to an off-line optical readout, without the need of enzyme inhibitors.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- Teknisk fysik med inriktning mot mikrosystemteknik
- Engineering Science with specialization in Microsystems Technology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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