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A Switch from Cell-...
A Switch from Cell-Associated to Soluble PDGF-B Protects against Atherosclerosis, despite Driving Extramedullary Hematopoiesis
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- Tillie, Renee J. H. A. (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.
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- Theelen, Thomas L. (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.
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- van Kuijk, Kim (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.
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- Temmerman, Lieve (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.
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- de Bruijn, Jenny (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.
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- Gijbels, Marion (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.;Maastricht Univ, Dept Pathol, GROW Sch Oncol & Dev Biol, Med Ctr, NL-6229 HX Maastricht, Netherlands.;Univ Amsterdam, Expt Vasc Biol, Dept Med Biochem, Amsterdam UMC, NL-1105 AZ Amsterdam, Netherlands.
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- Betsholtz, Christer (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Biessen, Erik A. L. (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.;Rhein Westfal TH Aachen, Inst Mol Cardiovasc Res IMCAR, D-52074 Aachen, Germany.
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- Sluimer, Judith C. (författare)
- Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.;Univ Edinburgh, BHF Ctr Cardiovasc Sci CVS, Edinburgh EH16 4TJ, Midlothian, Scotland.
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Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands Maastricht Univ, Cardiovasc Res Inst Maastricht CARIM, Dept Pathol, Med Ctr, NL-6229 HX Maastricht, Netherlands.;Maastricht Univ, Dept Pathol, GROW Sch Oncol & Dev Biol, Med Ctr, NL-6229 HX Maastricht, Netherlands.;Univ Amsterdam, Expt Vasc Biol, Dept Med Biochem, Amsterdam UMC, NL-1105 AZ Amsterdam, Netherlands. (creator_code:org_t)
- 2021-07-10
- 2021
- Engelska.
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Ingår i: Cells. - : MDPI. - 2073-4409. ; 10:7, s. 1746-
- Relaterad länk:
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Platelet-derived growth factor B (PDGF-B) is a mitogenic, migratory and survival factor. Cell-associated PDGF-B recruits stabilizing pericytes towards blood vessels through retention in extracellular matrix. We hypothesized that the genetic ablation of cell-associated PDGF-B by retention motif deletion would reduce the local availability of PDGF-B, resulting in microvascular pericyte loss, microvascular permeability and exacerbated atherosclerosis. Therefore, Ldlr(-/-)Pdgfb(ret)(/ret) mice were fed a high cholesterol diet. Although plaque size was increased in the aortic root of Pdgfb(ret)(/ret) mice, microvessel density and intraplaque hemorrhage were unexpectedly unaffected. Plaque macrophage content was reduced, which is likely attributable to increased apoptosis, as judged by increased TUNEL+ cells in Pdgfb(ret)(/ret) plaques (2.1-fold) and increased Pdgfb(ret)(/ret) macrophage apoptosis upon 7-ketocholesterol or oxidized LDL incubation in vitro. Moreover, Pdgfb(ret)(/ret) plaque collagen content increased independent of mesenchymal cell density. The decreased macrophage matrix metalloproteinase activity could partly explain Pdgfb(ret)(/ret) collagen content. In addition to the beneficial vascular effects, we observed reduced body weight gain related to smaller fat deposition in Pdgfb(ret)(/ret) liver and adipose tissue. While dampening plaque inflammation, Pdgfb(ret)(/ret) paradoxically induced systemic leukocytosis. The increased incorporation of 5-ethynyl-2 '-deoxyuridine indicated increased extramedullary hematopoiesis and the increased proliferation of circulating leukocytes. We concluded that Pdgfb(ret)(/ret) confers vascular and metabolic effects, which appeared to be protective against diet-induced cardiovascular burden. These effects were unrelated to arterial mesenchymal cell content or adventitial microvessel density and leakage. In contrast, the deletion drives splenic hematopoiesis and subsequent leukocytosis in hypercholesterolemia.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- PDGF-B
- atherosclerosis
- plaque stability
- fibrosis
- hematopoiesis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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