UPLC-QToF Nanospray MS and NMR Analysis of Ficus sycomorus Stem Bark and Its Effects on Rabbit

• In the present study, a phytochemical of Ficus sycomorus (Moraceae family) was screened, and the effect of this extract on rabbit performance indices, immunity, and carcass quality measures was determined. Ficus sycomorus samples were collected, air-dried, and extracted with 70% methanol to prepare a solution of 100 mg/mL concentration. The extract was subjected to high-resolution mass spectrometric measurements via ultra-high performance liquid chromatography-quadrupole time-of-flight-nanospray mass spectrometry (UPLC-QToF-MS) and 1H NMR analysis. Forty-eight male rabbits, one-month-old, belonging to the Blanc de Bouscat and New Zealand White breeds were selected and distributed equally in a 2 x 3 factorial trial. The rabbits within each breed received F. sycomorus extract at the dose of 0, 100, and 200 mg/kg for 60 days. Blood samples were collected and serum obtained for the detection of liver enzymes, serum lipids, and proteins. The results of UPLC-QToF-MS and molecular networking analysis revealed the presence of procyanidin B2, procyanidin A1, genistein, eriodyctiol, catechin, luteolin, biochanin A, and chlorogenic acid that might exhibit various pharmaceutical activities. However, the F. sycomorus extract reduced rabbit performance indices and carcass quality measures. In addition, this extract significantly depressed the low-density lipoprotein and triglycerides, which may indicate the antidyslipidemia effect of this extract on rabbits.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Nyckelord

Ficus sycomorus

UPLC-QToF nanospray MS

NMR analysis

antidyslipidemia effect

rabbits

Publikations- och innehållstyp

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