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Kinetic Analysis Su...
Kinetic Analysis Suggests Evolution of Ribosome Snecificity in Modern Elongation Factor-Tus from "Generalist" Ancestors
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- De Tarafder, Arindam (författare)
- Uppsala universitet,Molekylärbiologi
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- Parajuli, Narayan Prasad (författare)
- Uppsala universitet,Molekylärbiologi
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- Majumdar, Soneya (författare)
- Uppsala universitet,Molekylärbiologi
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- Kacar, Betul (författare)
- Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA.;Univ Arizona, Lunar & Planetary Lab, Tucson, AZ 85721 USA.;Univ Arizona, Steward Observ, Tucson, AZ USA.
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- Sanyal, Suparna (författare)
- Uppsala universitet,Teknisk-naturvetenskapliga fakulteten,Molekylärbiologi
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(creator_code:org_t)
- 2021-04-19
- 2021
- Engelska.
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Ingår i: Molecular biology and evolution. - : Oxford University Press. - 0737-4038 .- 1537-1719. ; 38:8, s. 3436-3444
- Relaterad länk:
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https://doi.org/10.1...
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- It has been hypothesized that early enzymes are more promiscuous than their extant orthologs. Whether or not this hypothesis applies to the translation machinery, the oldest molecular machine of life, is not known. Efficient protein synthesis relies on a cascade of specific interactions between the ribosome and the translation factors. Here, using elongation factor-Tu (EF-Tu) as a model system, we have explored the evolution of ribosome specificity in translation factors. Employing presteady state fast kinetics using quench flow, we have quantitatively characterized the specificity of two sequence-reconstructed 1.3- to 3.3-Gy-old ancestral EF-Tus toward two unrelated bacterial ribosomes, mesophilic Escherichia coil and thermophilic Thermus thermophilus. Although the modern EF-Tus show clear preference for their respective ribosomes, the ancestral EF-Tus show similar specificity for diverse ribosomes. In addition, despite increase in the catalytic activity with temperature, the ribosome specificity of the thermophilic EF-Tus remains virtually unchanged. Our kinetic analysis thus suggests that EF-Tu proteins likely evolved from the catalytically promiscuous, "generalist" ancestors. Furthermore, compatibility of diverse ribosomes with the modern and ancestral EF-Tus suggests that the ribosomal core probably evolved before the diversification of the EF-Tus. This study thus provides important insights regarding the evolution of modern translation machinery.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- translation machinery
- molecular evolution
- EF-Tu
- generalist
- ancestral sequence reconstruction
- fast kinetics
- specificity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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