Sökning: onr:"swepub:oai:DiVA.org:uu-456854" >
The citrullinated/n...
The citrullinated/native index of autoantibodies against hnRNP-DL predicts an individual "window of treatment success" in RA patients
-
- Marklein, Bianka (författare)
- Charite Univ Med Berlin, Dept Rheumatol & Clin Immunol, Charite Campus Mitte, Rheumatol Forschungslab AG Skriner, Charitepl 1,Intern Virchowweg 11,5-OG,R011, D-10117 Berlin, Germany
-
- Jenning, Madeleine (författare)
- Charite Univ Med Berlin, Dept Rheumatol & Clin Immunol, Charite Campus Mitte, Rheumatol Forschungslab AG Skriner, Charitepl 1, Intern Virchowweg 11, 5-OG, R011, D-10117 Berlin, Germany; Leibniz Inst, German Rheumatism Res Ctr, D-10117 Berlin, Germany
-
- Konthur, Zoltán (författare)
- Max Planck Inst Mol Genet, Berlin, Germany; Max Planck Inst Colloids & Interfaces, Potsdam, Germany; Bundesanstalt Mat Forsch & Prufung BAM, Dept Analyt Chem, Dept 1, Berlin, Germany
-
visa fler...
-
- Häupl, Thomas (författare)
- Charite Univ Med Berlin, Dept Rheumatol & Clin Immunol, Charite Campus Mitte, Rheumatol Forschungslab AG Skriner, Charitepl 1,Intern Virchowweg 11,5-OG,R011, D-10117 Berlin, Germany
-
- Welzel, Franziska (författare)
- Max Planck Inst Mol Genet, Berlin, Germany
-
- Nonhoff, Ute (författare)
- Max Planck Inst Mol Genet, Berlin, Germany
-
- Krobitsch, Sylvia (författare)
- Max Planck Inst Mol Genet, Berlin, Germany
-
- Mulder, Debbie M. (författare)
- Radboud Univ Nijmegen, Dept Expt Rheumatol, Med Ctr, Nijmegen, Netherlands
-
- Koenders, Marije I. (författare)
- Radboud Univ Nijmegen, Dept Expt Rheumatol, Med Ctr, Nijmegen, Netherlands
-
- Joshua, Vijay (författare)
- Karolinska Institutet
-
- Cope, Andrew P. (författare)
- Kings Coll London, Fac Life Sci & Med, Ctr Rheumat Dis, Sch Immunol & Microbial Sci, London, England
-
- Shlomchik, Mark J. (författare)
- Univ Pittsburgh, Dept Immunol, Sch Med, Pittsburgh, PA USA
-
- Anders, Hans-Joachim (författare)
- Ludwig Maximilian Univ Hosp, Nephrol Ctr, Med Clin & Policlin 4, Munich, Germany
-
- Burmester, Gerd R. (författare)
- Charite Univ Med Berlin, Dept Rheumatol & Clin Immunol, Charite Campus Mitte, Rheumatol Forschungslab AG Skriner, Charitepl 1,Intern Virchowweg 11,5-OG,R011, D-10117 Berlin, Germany
-
- Hensvold, Aase (författare)
- Karolinska Institutet
-
- Catrina, Anca I. (författare)
- Karolinska Inst, Karolinska Univ Hosp, Dept Med Solna, Div Rheumatol, Stockholm, Sweden
-
- Rönnelid, Johan (författare)
- Uppsala universitet,Klinisk immunologi
-
- Steiner, Günter (författare)
- Med Univ Vienna, Div Rheumatol, Vienna, Austria; Ludwig Boltzmann Cluster Arthrit & Rehabil, Vienna, Austria
-
- Skriner, Karl (författare)
- Charite Univ Med Berlin, Dept Rheumatol & Clin Immunol, Charite Campus Mitte, Rheumatol Forschungslab AG Skriner, Charitepl 1, Intern Virchowweg 11, 5-OG, R011, D-10117 Berlin, Germany; Leibniz Inst, German Rheumatism Res Ctr, D-10117 Berlin, Germany
-
visa färre...
-
(creator_code:org_t)
- 2021-09-14
- 2021
- Engelska.
-
Ingår i: Arthritis Research & Therapy. - : BioMed Central (BMC). - 1478-6362. ; 23:1
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://arthritis-re...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- BackgroundThere is a need for biomarker to identify patients “at risk” for rheumatoid arthritis (risk-RA) and to better predict the therapeutic response and in this study we tested the hypothesis that novel native and citrullinated heterogeneous nuclear ribonucleoprotein (hnRNP)-DL autoantibodies could be possible biomarkers.MethodsUsing protein macroarray and ELISA, epitope recognition against hnRNP-DL was analysed in sera from different developed RA disease and diagnosed SLE patients. Toll-like receptor (TLR) 7/9 and myeloid differentiation primary response gene 88 (MyD88)-dependency were studied in sera from murine disease models. HnRNP-DL expression in cultivated cells and synovial tissue was analysed by indirect immunofluorescence, immunoblot and immunohistochemistry.ResultsHnRNP-DL was highly expressed in stress granules, citrullinated in the rheumatoid joint and targeted by autoantibodies either as native or citrullinated proteins in patient subsets with different developed RA disease. Structural citrullination dependent epitopes (SCEs) of hnRNP-DL were detected in 58% of the SLE patients although 98% of these sera were α-CCP-2-negative. To obtain a specific citrullinated signal value, we subtracted the native antibody value from the citrullinated signal. The citrullinated/native index of autoantibodies against hnRNP-DL (CNDL-Index) was identified as a new value for an “individual window of treatment success” in early RA and for the detection of RF IgM/α-CCP-2 seronegative RA patients (24–46%). Negative CNDL-index was found in SLE patients, risk-RA and early RA cohorts such as EIRA where the majority of these patients are DAS28-responders to methotrexate (MTX) treatment (87%). High positive CNDL-values were associated with more severe RA, shared epitope and parenchymal changes in the lung. Specifically, native α-hnRNP-DL is TLR7/9-dependent, associated with pain and ROC analysis revealed an association to initial MTX or etanercept treatment response, especially in seronegative RA patients.ConclusionCNDL-index defines people at risk to develop RA and the “window of treatment success” thereby closing the sensitivity gap in RA.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
Nyckelord
- Rheumatoid arthritis
- ACPA
- Anti-CCP
- Rheumatoid factor
- Shared epitope
- Systemic lupus erythematosus
- Autoantigens
- Treatment
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Marklein, Bianka
-
Jenning, Madelei ...
-
Konthur, Zoltán
-
Häupl, Thomas
-
Welzel, Franzisk ...
-
Nonhoff, Ute
-
visa fler...
-
Krobitsch, Sylvi ...
-
Mulder, Debbie M ...
-
Koenders, Marije ...
-
Joshua, Vijay
-
Cope, Andrew P.
-
Shlomchik, Mark ...
-
Anders, Hans-Joa ...
-
Burmester, Gerd ...
-
Hensvold, Aase
-
Catrina, Anca I.
-
Rönnelid, Johan
-
Steiner, Günter
-
Skriner, Karl
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Reumatologi och ...
- Artiklar i publikationen
-
Arthritis Resear ...
- Av lärosätet
-
Uppsala universitet
-
Karolinska Institutet