Sökning: onr:"swepub:oai:DiVA.org:uu-457474" >
Recombinant antibod...
Recombinant antibody against Trypanosoma cruzi from patients with chronic Chagas heart disease recognizes mammalian nervous system
-
- Niborski, Leticia L. (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina.;PSL Res Univ, Inst Curie, Translmm Team, INSEAM U932, Paris, France.
-
- Potenza, Mariana (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina.
-
- Chirivi, Renato G. S. (författare)
- ModiQuest BV, Oss, Netherlands.
-
visa fler...
-
- Simonetti, Leandro (författare)
- Uppsala universitet,Biokemi
-
- Ossowski, Micaela S. (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina.
-
- Grippo, Vanina (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina.;Consejo Nacl Invest Cient & Tecn, ICT Milstein, Ctr Virol Anim CEVAN, Buenos Aires, DF, Argentina.
-
- May, Maria (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Biol & Med Expt IBYME, Buenos Aires, DF, Argentina.
-
- Staquicini, Daniela, I (författare)
- Univ Fed Sao Paulo, Dept Microbiol Inmunol & Parasitol, Escola Paulista Med, Sao Paulo, SP, Brazil.;Rutgers Canc Inst New Jersey, Newark, NJ USA.;Rutgers New Jersey Med Sch, Dept Radiat Oncol, Div Canc Biol, Newark, NJ USA.
-
- Parodi-Talice, Adriana (författare)
- Inst Pasteur Montevideo, Unidad Biol Mol, Montevideo, Uruguay.;Univ Republica, Fac Ciencias, Secc Genet, Montevideo, Uruguay.
-
- Robello, Carlos (författare)
- Inst Pasteur Montevideo, Unidad Biol Mol, Montevideo, Uruguay.;Univ Republica, Fac Med, Dept Bioquim, Montevideo, Uruguay.
-
- Comini, Marcelo A. (författare)
- Inst Pasteur Montevideo, Grp Redox Biol Trypanosomes, Montevideo, Uruguay.
-
- Alonso, Guillermo D. (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina.
-
- Raats, Jos M. H. (författare)
- ModiQuest BV, Oss, Netherlands.
-
- Gomez, Karina A. (författare)
- Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina.
-
visa färre...
-
Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina;PSL Res Univ, Inst Curie, Translmm Team, INSEAM U932, Paris, France. Consejo Nacl Invest Cient & Tecn, Inst Invest Ingn Genet & Biol Mol INGEBI, Buenos Aires, DF, Argentina. (creator_code:org_t)
- Elsevier, 2021
- 2021
- Engelska.
-
Ingår i: EBioMedicine. - : Elsevier. - 2352-3964. ; 63
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
http://www.thelancet...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background: To deeply understand the role of antibodies in the context of Trypanosoma cruzi infection, we decided to characterize A2R1, a parasite antibody selected from single-chain variable fragment (scFv) phage display libraries constructed from B cells of chronic Chagas heart disease patients. Methods: Immunoblot, ELISA, cytometry, immunofluorescence and immunohistochemical assays were used to characterize A2R1 reactivity. To identify the antibody target, we performed an immunoprecipitation and two-dimensional electrophoresis coupled to mass spectrometry and confirmed A2R1 specific interaction by producing the antigen in different expression systems. Based on these data, we carried out a comparative in silico analysis of the protein targets orthologues, focusing mainly on post-translational modifications. Findings: A2R1 recognizes a parasite protein of similar to 50 kDa present in all life cycle stages of T. cruzi, as well as in other members of the kinetoplastid family, showing a defined immunofluorescence labeling pattern consistent with the cytoskeleton. A2R1 binds to tubulin, but this interaction relies on its post-translational modifications. Interestingly, this antibody also targets mammalian tubulin only present in brain, staining in and around cell bodies of the human peripheral and central nervous system. Interpretation: Our findings demonstrate for the first time the existence of a human antibody against T. cruzi tubulin capable of cross-reacting with a human neural protein. This work re-emphasizes the role of molecular mimicry between host and parasitic antigens in the development of pathological manifestations of T. cruzi infection.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Chagas disease
- Phage-display
- Tubulin
- Molecular mimicry
- Post-translational modification
- Digestive system
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Niborski, Letici ...
-
Potenza, Mariana
-
Chirivi, Renato ...
-
Simonetti, Leand ...
-
Ossowski, Micael ...
-
Grippo, Vanina
-
visa fler...
-
May, Maria
-
Staquicini, Dani ...
-
Parodi-Talice, A ...
-
Robello, Carlos
-
Comini, Marcelo ...
-
Alonso, Guillerm ...
-
Raats, Jos M. H.
-
Gomez, Karina A.
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
EBioMedicine
- Av lärosätet
-
Uppsala universitet