onr:"swepub:oai:DiVA.org:uu-457888"
Sökning: onr:"swepub:oai:DiVA.org:uu-457888" > ZBED6 regulates Igf...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04377naa a2200445 4500 | |
| 001 | oai:DiVA.org:uu-457888 | |
| 003 | SwePub | |
| 008 | 211108s2021 | |||||||||||000 ||eng| | |
| 009 | oai:slubar.slu.se:114071 | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4578882 URI |
| 024 | 7 | a https://doi.org/10.1038/s41598-021-98777-02 DOI |
| 024 | 7 | a https://res.slu.se/id/publ/1140712 URI |
| 040 | a (SwePub)uud (SwePub)slu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Naboulsi, Rakanu Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)rakna705 |
| 245 | 1 0 | a ZBED6 regulates Igf2 expression partially through its regulation of miR483 expression |
| 264 | c 2021-09-30 | |
| 264 | 1 | b Springer Nature,c 2021 |
| 338 | a electronic2 rdacarrier | |
| 520 | a The expression of Igf2 in mammals shows a complex regulation involving multiple promoters and epigenetic mechanisms. We previously identified a novel regulatory mechanism based on the interaction between the transcriptional factor ZBED6 and Igf2 intron. Disruption of the ZBED6-Igf2 interaction leads to a dramatic up-regulation of IGF2 expression postnatally. In the current study we characterize an additional layer of regulation involving miR483 encoded by another Igf2 intron. We found a highly significant up-regulation of miR483 expression when the ZBED6-Igf2 axis is disrupted in transgenic mice. Furthermore, CRISPR/Cas9 mediated knock-out of miR483 in C2C12 myoblast cells, both wild-type and cells with disrupted ZBED6-Igf2 axis (Igf2(dGGCT)), resulted in down-regulation of Igf2 expression and a reduced proliferation rate. This was further validated using miR483 mimics and inhibitors. RNA-seq analysis revealed a significant enrichment of genes involved in the PI3K-Akt signaling pathway among genes down-regulated in miR483(-/-) cells, including Igf2 down-regulation. The opposite pattern was observed in Igf2(dGGCT) cells, where Igf2 is up-regulated. Our data suggest a positive feedback between miR483 and Igf2 promoter activity, strongly affecting how ZBED6 controls Igf2 expression in various cell types. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Cellbiologi0 (SwePub)106042 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Cell Biology0 (SwePub)106042 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Utvecklingsbiologi0 (SwePub)106142 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Developmental Biology0 (SwePub)106142 hsv//eng |
| 700 | 1 | a Larsson, Mårtenu Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)mla24680 |
| 700 | 1 | a Andersson, Leifu Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab,Swedish Univ Agr Sci, Dept Anim Breeding & Genet, S-75007 Uppsala, Sweden.;Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics,Uppsala University,Texas A&M University4 aut0 (Swepub:slu)48730 |
| 700 | 1 | a Younis, Shadyu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Ain Shams Univ, Dept Anim Breeding & Genet, Cairo 11241, Egypt.;Stanford Univ, Dept Med, Div Immunol & Rheumatol, Stanford, CA 94305 USA.4 aut0 (Swepub:uu)shayo804 |
| 710 | 2 | a Uppsala universitetb Institutionen för medicinsk biokemi och mikrobiologi4 org |
| 710 | 2 | a Sveriges lantbruksuniversitet |
| 773 | 0 | t Scientific Reportsd : Springer Natureg 11:1q 11:1x 2045-2322 |
| 856 | 4 | u https://doi.org/10.1038/s41598-021-98777-0y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1609295/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 | u https://www.nature.com/articles/s41598-021-98777-0.pdf |
| 856 | 4 | u https://pub.epsilon.slu.se/id/eprint/25955/contentsx primaryx Raw objectx freey FULLTEXT |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-457888 |
| 856 | 4 8 | u https://doi.org/10.1038/s41598-021-98777-0 |
| 856 | 4 8 | u https://res.slu.se/id/publ/114071 |
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