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A UPLC-DAD-Based Bi...
A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds : Pyrroquinazoline Alkaloids from Adhatoda vasica as a Case Study
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- Tehreem, Syeda (författare)
- Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan.
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- Rahman, Saeedur (författare)
- Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan.
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- Bhatti, Muhammad Salman (författare)
- Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan.
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- Uddin, Reaz (författare)
- Univ Karachi, Dr Panjwani Ctr Mol Med & Drug Res, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan.
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- Khan, Muhammad Noman (författare)
- Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan.
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- Tauseef, Saba (författare)
- Univ Karachi, Dr Panjwani Ctr Mol Med & Drug Res, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan.
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- El-Seedi, Hesham (författare)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Jiangsu, Peoples R China.,Farmakognosi
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- Bin Muhsinah, Abdullatif (författare)
- King Khalid Univ, Coll Pharm, Dept Pharmacognosy, Abha 62529, Saudi Arabia.
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- Uddin, Jalal (författare)
- King Khalid Univ, Coll Pharm, Dept Pharmaceut Chem, Abha 62529, Saudi Arabia.
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- Musharraf, Syed Ghulam (författare)
- Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan.;Univ Karachi, Dr Panjwani Ctr Mol Med & Drug Res, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan.;Southwest Med Univ, TCM Hosp, Luzhou 646000, Peoples R China.
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Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan Univ Karachi, Dr Panjwani Ctr Mol Med & Drug Res, Int Ctr Chem & Biol Sci, Karachi 75270, Pakistan. (creator_code:org_t)
- 2021-11-18
- 2021
- Engelska.
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Ingår i: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 26:22
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Angiotensin converting enzyme (ACE) plays a crucial role in regulating blood pressure in the human body. Identification of potential ACE inhibitors from medicinal plants supported the idea of repurposing these medicinal plants against hypertension. A method based on ultra-performance liquid chromatography (UPLC) coupled with a diode array detector (DAD) was used for the rapid screening of plant extracts and purified compounds to determine their ACE inhibitory activity. Hippuryl-histidiyl-leucine (HHL) was used as a substrate, which is converted into hippuric acid (HA) by the action of ACE. A calibration curve of the substrate HHL was developed with the linear regression 0.999. The limits of detection and quantification of this method were found to be 0.134 and 0.4061 mM, respectively. Different parameters of ACE inhibitory assay were optimized, including concentration, incubation time and temperature. The ACE inhibition potential of Adhatoda vasica (methanolic-aqueous extract) and its isolated pyrroquinazoline alkaloids, vasicinol (1), vasicine (2) and vasicinone (3) was evaluated. Compounds 1-3 were characterized by various spectroscopic techniques. The IC50 values of vasicinol (1), vasicine (2) and vasicinone (3) were found to be 6.45, 2.60 and 13.49 mM, respectively. Molecular docking studies of compounds 1-3 were also performed. Among these compounds, vasicinol (1) binds as effectively as captopril, a standard drug of ACE inhibition.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- angiotensin converting enzyme
- hypertension
- pyrroquinazoline alkaloids
- vasicine
- vasicinol
- vasicinone
Publikations- och innehållstyp
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Till lärosätets databas
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Tehreem, Syeda
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Rahman, Saeedur
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Bhatti, Muhammad ...
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Uddin, Reaz
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Khan, Muhammad N ...
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Tauseef, Saba
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El-Seedi, Hesham
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Bin Muhsinah, Ab ...
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Uddin, Jalal
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Musharraf, Syed ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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Molecules
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Uppsala universitet