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Mapping the thermor...
Mapping the thermoresponses of micralgae by integrating single-cell arrays on a programmable temperature stage
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- Johansson, Sofia (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Mikrosystemteknik,EMBLA
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- Xiao, Linhong (författare)
- Uppsala universitet,Miljötoxikologi,Science for Life Laboratory, SciLifeLab
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- Andersson, Martin (författare)
- Uppsala universitet,Institutionen för materialvetenskap,Science for Life Laboratory, SciLifeLab,EMBLA
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- Bergman, Henrik (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för materialvetenskap
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- Behrendt, Lars (författare)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Miljötoxikologi
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- Tenje, Maria (författare)
- Uppsala universitet,Mikrosystemteknik,Science for Life Laboratory, SciLifeLab,Department of Materials Science and Engineering, Science for Life Laboratory, Uppsala University, Uppsala, Sweden,EMBLA
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(creator_code:org_t)
- 2021
- 2021
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- We report on a miniaturized temperature regulation system for the precise control of thermal environments inside microfluidic devices. This system uses a combination of Joule heating and active cooling to achieve defined temperatures to an affixed microfluidic device, allowing for rapid and cost-effective temperature control in single-use devices. Spatiotemporal temperature profiles in a biologically relevant temperature range (20-40 °C) are programmed in a custom software. We illustrate the capabilities of the system with a study of the coral symbiont Symbiodinium at elevated temperatures. Through monitoring of single-cell photophysiology, we evaluate the response to different applied temperature profile and the strain-specific thermal sensitivity thresholds.
Nyckelord
- thermal stress
- Symbiodinium
- temperature regulation
- single-cell
- Joule heating
- Teknisk fysik med inriktning mot mikrosystemteknik
- Engineering Science with specialization in Microsystems Technology
Publikations- och innehållstyp
- vet (ämneskategori)
- kon (ämneskategori)