Sökning: onr:"swepub:oai:DiVA.org:uu-472756" > Inflammation and ne...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 06018naa a2200601 4500 | |
001 | oai:DiVA.org:uu-472756 | |
003 | SwePub | |
008 | 220419s2022 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4727562 URI |
024 | 7 | a https://doi.org/10.1007/s00018-022-04224-22 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Yau, Anthony C. Y.u Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)antya284 |
245 | 1 0 | a Inflammation and neutrophil extracellular traps in cerebral cavernous malformation |
264 | c 2022-03-25 | |
264 | 1 | b Springer Nature,c 2022 |
338 | a electronic2 rdacarrier | |
520 | a Cerebral Cavernous Malformation (CCM) is a brain vascular disease with various neurological symptoms. In this study, we describe the inflammatory profile in CCM and show for the first time the formation of neutrophil extracellular traps (NETs) in rodents and humans with CCM. Through RNA-seq analysis of cerebellum endothelial cells from wild-type mice and mice with an endothelial cell-specific ablation of the Ccm3 gene (Ccm3(iECKO)), we show that endothelial cells from Ccm3(iECKO) mice have an increased expression of inflammation-related genes. These genes encode proinflammatory cytokines and chemokines, as well as adhesion molecules, which promote recruitment of inflammatory and immune cells. Similarly, immunoassays showed elevated levels of these cytokines and chemokines in the cerebellum of the Ccm3(iECKO) mice. Consistently, both flow cytometry and immunofluorescence analysis showed infiltration of different subsets of leukocytes into the CCM lesions. Neutrophils, which are known to fight against infection through different strategies, including the formation of NETs, represented the leukocyte subset within the most pronounced increase in CCM. Here, we detected elevated levels of NETs in the blood and the deposition of NETs in the cerebral cavernomas of Ccm3(iECKO) mice. Degradation of NETs by DNase I treatment improved the vascular barrier. The deposition of NETs in the cavernomas of patients with CCM confirms the clinical relevance of NETs in CCM. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
653 | a Inflammation | |
653 | a Neutrophil extracellular traps | |
653 | a Endothelial cells | |
653 | a Cerebral cavernous malformations | |
700 | 1 | a Globisch, Maria A.u Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)margl506 |
700 | 1 | a Onyeogaziri, Favouru Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)favon830 |
700 | 1 | a Conze, Lei Liuu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut0 (Swepub:uu)leliu102 |
700 | 1 | a Smith, Ross O.u Uppsala universitet,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)rossm781 |
700 | 1 | a Jauhiainen, Suviu Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)suvja782 |
700 | 1 | a Corada, Monicau FIRC Inst Mol Oncol Fdn, Vasc Biol Unit, Milan, Italy.4 aut |
700 | 1 | a Orsenigo, Fabriziou FIRC Inst Mol Oncol Fdn, Vasc Biol Unit, Milan, Italy.4 aut |
700 | 1 | a Huang, Hua,d 1986-u Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)huahu114 |
700 | 1 | a Herre, Melanieu Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)melhe672 |
700 | 1 | a Olsson, Anna-Karinu Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:uu)annaolss |
700 | 1 | a Malinverno, Matteou FIRC Inst Mol Oncol Fdn, Vasc Biol Unit, Milan, Italy.4 aut |
700 | 1 | a Sundell, Veronicau Uppsala universitet,Institutionen för immunologi, genetik och patologi4 aut0 (Swepub:uu)versu224 |
700 | 1 | a Jahromi, Behnam Rezaiu Univ Helsinki, Dept Neurosurg, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland.4 aut |
700 | 1 | a Niemela, Mikau Univ Helsinki, Dept Neurosurg, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland.4 aut |
700 | 1 | a Laakso, Akiu Univ Helsinki, Dept Neurosurg, Helsinki, Finland.;Helsinki Univ Hosp, Helsinki, Finland.4 aut |
700 | 1 | a Garlanda, Ceciliau Humanitas Univ, Dept Biomed Sci, Milan, Italy.;IRCCS Humanitas Res Hosp, Milan, Italy.4 aut |
700 | 1 | a Mantovani, Albertou Humanitas Univ, Dept Biomed Sci, Milan, Italy.;IRCCS Humanitas Res Hosp, Milan, Italy.;Queen Mary Univ London, William Harvey Res Inst, London, England.4 aut |
700 | 1 | a Lampugnani, Maria Graziau FIRC Inst Mol Oncol Fdn, Vasc Biol Unit, Milan, Italy.;Mario Negri Inst Pharmacol Res, I-20157 Milan, Italy.4 aut |
700 | 1 | a Dejana, Elisabettau Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab,FIRC Inst Mol Oncol Fdn, Vasc Biol Unit, Milan, Italy.4 aut0 (Swepub:uu)elide443 |
700 | 1 | a Magnusson, Peetrau Uppsala universitet,Science for Life Laboratory, SciLifeLab,Klinisk immunologi4 aut0 (Swepub:uu)petrmagn |
710 | 2 | a Uppsala universitetb Institutionen för immunologi, genetik och patologi4 org |
773 | 0 | t Cellular and Molecular Life Sciences (CMLS)d : Springer Natureg 79:4q 79:4x 1420-682Xx 1420-9071 |
856 | 4 | u https://doi.org/10.1007/s00018-022-04224-2y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1652317/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-472756 |
856 | 4 8 | u https://doi.org/10.1007/s00018-022-04224-2 |
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