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Bovine oocyte expos...
Bovine oocyte exposure to perfluorohexane sulfonate (PFHxS) induces phenotypic, transcriptomic, and DNA methylation changes in resulting embryos in vitro
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- Hallberg, Ida (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
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- Persson, Sara (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Swedish Museum of Natural History
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- Olovsson, Matts, 1958- (författare)
- Uppsala universitet,Reproduktionsbiologi,Ctr Reprod Biol Uppsala, SE-75237 Uppsala, Sweden.
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- Moberg, Mikaela (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
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- Ranefall, Petter, 1968- (författare)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab
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- Laskowski, Denise (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences
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- Damdimopoulou, Pauliina (författare)
- Karolinska Institutet
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- Sirard, Marc-Andre (författare)
- Laval Univ, Dept Anim Sci, Quebec City, PQ G1V 0A6, Canada.
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- Rüegg, Joëlle (författare)
- Uppsala universitet,Miljötoxikologi,Ctr Reprod Biol Uppsala, SE-75236 Uppsala, Sweden.
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- Sjunnesson, Ylva C. B. (författare)
- Swedish Univ Agr Sci, Ctr Reprod Biol Uppsala, Dept Clin Sci, Div Reprod, SE-75007 Uppsala, Sweden.
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(creator_code:org_t)
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- Elsevier, 2022
- 2022
- Engelska.
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Ingår i: Reproductive Toxicology. - : Elsevier. - 0890-6238 .- 1873-1708. ; 109, s. 19-30
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Abstract
Ämnesord
Stäng
- Knowledge on the effects of perfluorohexane sulfonate (PFHxS) on ovarian function is limited. In the current study, we investigated the sensitivity of oocytes to PFHxS during in vitro maturation (IVM), including conse-quences on embryo development at the morphological, transcriptomic, and epigenomic levels. Bovine cumulus-oocyte complexes (COCs) were exposed to PFHxS during 22 h IVM. Following fertilisation, developmental competence was recorded until day 8 of culture. Two experiments were conducted: 1) exposure of COCs to 0.01 mu g mL(-1) -100 mu g mL(-1) PFHxS followed by confocal imaging to detect neutral lipids and nuclei, and 2) exposure of COCs to 0.1 mu g mL(-1) PFHxS followed by analysis of transcriptomic and DNA methylation changes in blastocysts. Decreased oocyte developmental competence was observed upon exposure to & nbsp;>= 40 mu g mL(-1) PFHxS and altered lipid distribution was observed in the blastocysts upon exposure to 1-10 mu g mL(-1) PFHxS (not observed at lower or higher concentrations). Transcriptomic data showed that genes affected by 0.1 mu g mL(-1) PFHxS were enriched for pathways related to increased synthesis and production of reactive oxygen species. Enrichment for peroxisome proliferator-activated receptor-gamma and oestrogen pathways was also observed. Genes linked to DNA methylation changes were enriched for similar pathways. In conclusion, exposure of the bovine oocyte to PFHxS during the narrow window of IVM affected subsequent embryonic development, as reflected by morphological and mo- lecular changes. This suggests that PFHxS interferes with the final nuclear and cytoplasmic maturation of the oocyte leading to decreased developmental competence to blastocyst stage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- Per- and polyfluoroalkyl substances
- PFAS & nbsp
- Female fertility & nbsp
- Bovine
- IVP
- Oocyte maturation
- Embryo quality & nbsp
- Embryo quality Reproductive toxicity
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Hallberg, Ida
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Persson, Sara
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Olovsson, Matts, ...
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Moberg, Mikaela
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Ranefall, Petter ...
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Laskowski, Denis ...
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visa fler...
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Damdimopoulou, P ...
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Sirard, Marc-And ...
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Rüegg, Joëlle
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Sjunnesson, Ylva ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Reproduktionsmed ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Farmakologi och ...
- Artiklar i publikationen
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Reproductive Tox ...
- Av lärosätet
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Uppsala universitet
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Karolinska Institutet
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Sveriges Lantbruksuniversitet