Sökning: onr:"swepub:oai:DiVA.org:uu-474373" > Evaluation of an ex...
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000 | 04730naa a2200517 4500 | |
001 | oai:DiVA.org:uu-474373 | |
003 | SwePub | |
008 | 220512s2022 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:235467176 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4743732 URI |
024 | 7 | a https://doi.org/10.1186/s40635-022-00443-w2 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:2354671762 URI |
040 | a (SwePub)uud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Skorup, Paulu Uppsala universitet,Infektionssjukdomar,Infektionsmedicin4 aut0 (Swepub:uu)pausk660 |
245 | 1 0 | a Evaluation of an extracorporeal ozone-based bactericide system for the treatment of Escherichia coli sepsis |
264 | c 2022-04-25 | |
264 | 1 | b Springer Nature,c 2022 |
338 | a electronic2 rdacarrier | |
520 | a Background: Sepsis is associated with substantial mortality rates. Antibiotic treatment is crucial, but global antibiotic resistance is now classified as one of the top ten global public health risks facing humanity. Ozone (O-3) is an inorganic molecule with no evident function in the body. We investigated the bactericide properties of ozone, using a novel system of extracorporeal ozone blood treatment. We hypothesized that ozone would decrease the concentration of viable Escherichia coli (E. coli) in human whole blood and that the system would be technically feasible and physiologically tolerable in a clinically relevant model of E. coli sepsis in swine. Methods: The E. coli strain B09-11822, a clinical isolate from a patient with septic shock was used. The in vitro study treated E. coli infected human whole blood (n = 6) with ozone. The in vivo 3.5-h sepsis model randomized swine to E. coli infusion and ozone treatment (n = 5) or E. coli infusion and no ozone treatment (n = 5). Live E. coli, 5 x 10(7) colony-forming units (CFU/mL) was infused in a peripheral vein. Ozone treatment was initiated with a duration of 30 min after 1.5 h. Results: The single pass in vitro treatment decreased E. coli by 27%, mean 1941 to 1422 CFU/mL, mean of differences - 519.0 (95% CI - 955.0 to - 82.98,P= 0.0281). pO(2) increased (95% CI 31.35 to 48.80, P= 0.0007), pCO(2) decreased (95% CI -3.203 to - 1.134, P= 0.0069), oxyhemoglobin increased (95% CI 1.010 to 3.669, P= 0.0113). Methemoglobin was not affected. In the sepsis model, 9/10 swine survived. One swine randomized to ozone treatment died from septic shock before initiation of the treatment. Circulatory, respiratory, and metabolic parameters were not affected by the ozone treatment. E. coli in arterial blood, in organs and in aerobic and anaerobic blood cultures did not differ. Hemoglobin, leucocytes, and methemoglobin were not affected by the treatment. Conclusions: Ozone decreased the concentration of viable E. coli in human whole blood. The system was technically feasible and physiologically tolerable in porcine sepsis/septic shock and should be considered for further studies towards clinical applications. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng |
653 | a Porcine sepsis model | |
653 | a Ozone | |
653 | a E. coli | |
653 | a Antibiotic resistance | |
653 | a Intensive care | |
700 | 1 | a Fransson, Anetteu Karolinska Inst, Dept Neurosci, Sect Expt Traumatol, Biomed 8B, S-17177 Stockholm, Sweden.4 aut |
700 | 1 | a Gustavsson, Jennyu Karolinska Institutet4 aut |
700 | 1 | a Sjoholm, Johanu Sangair AB, Stockholm, Sweden.4 aut |
700 | 1 | a Rundgren, Henriku Sangair AB, Stockholm, Sweden.4 aut |
700 | 1 | a ozenci, Volkanu Karolinska Institutet4 aut |
700 | 1 | a Wong, Alicia Y. W.u Karolinska Inst, Div Clin Microbiol, Dept Lab Med, Huddinge, Sweden.4 aut |
700 | 1 | a Karlsson, Tomasu Karolinska Institutet4 aut |
700 | 1 | a Svensen, Christeru Karolinska Institutet4 aut |
700 | 1 | a Gunther, Mattiasu Karolinska Institutet4 aut |
710 | 2 | a Uppsala universitetb Infektionssjukdomar4 org |
773 | 0 | t Intensive Care Medicine Experimentald : Springer Natureg 10:1q 10:1x 2197-425X |
856 | 4 | u https://doi.org/10.1186/s40635-022-00443-wy Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1657918/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-474373 |
856 | 4 8 | u https://doi.org/10.1186/s40635-022-00443-w |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:235467176 |
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