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An innovative dual ...
An innovative dual recognition aptasensor for specific detection of Staphylococcus aureus based on Au/Fe3O4 binary hybrid
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- El-Wekil, Mohamed M. (författare)
- Assiut Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Assiut 71524, Egypt
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- Halby, Hamada Mohamed (författare)
- Al Azhar Univ, Fac Pharm, Dept Microbiol & Immunol, Assiut Branch, Assiut 71524, Egypt
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- Darweesh, Mahmoud (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Al Azhar Univ, Fac Pharm, Dept Microbiol & Immunol, Assiut Branch, Assiut 71524, Egypt
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- Ali, Mohamed E. (författare)
- Al Azhar Univ, Fac Pharm, Dept Microbiol & Immunol, Assiut Branch, Assiut 71524, Egypt
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- Ali, Ramadan (författare)
- Al Azhar Univ, Fac Pharm, Dept Pharmaceut Analyt Chem, Assiut Branch, Assiut 71524, Egypt
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(creator_code:org_t)
- 2022-07-22
- 2022
- Engelska.
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Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 12:1
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Pathogenic bacteria cause disease outbreaks and threaten human health, prompting the research on advanced detection assays. Herein, we developed a selective molecular imprinted aptasensor for sensitive and prompt quantitation of Staphylococcus aureus (S. aureus) bacteria. The aptasensor was constructed by immobilization of aptamer on gold nanoparticles modified magnetic nanoparticles (apt-AuNPs@ Fe3O4). A functional monomer (o-phenylenediamine, o-phen) was electro-polymerized on the surface of the as-synthesized nanocomposite in the presence of a template (S. aureus). After removing S. aureus, the formed imprinted sites were available to extract pathogenic bacteria from complicated matrices. The surface morphology of the as-fabricated nanocomposites was characterized using different spectroscopic and electrochemical methods. Moreover, we thoroughly evaluated factors affecting the synthesis and determination procedures. The molecular imprinted aptasensor exhibited a wide linear range of 10(1)-10(7) CFU mL(-1) with a Limit of Detection, LOD (signal to noise = 3) of 1 CFU mL(-1). The aptasensor detected S. aureus in milk, conduit water, and apple juice samples with good recoveries % and satisfactory relative standard deviations (RSDs %) values.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
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