Sökning: onr:"swepub:oai:DiVA.org:uu-490822" >
Evaluation of RNA I...
Evaluation of RNA Isolation Methods in Human Adipose Tissue
-
Jui, Bipasha Nandi (författare)
-
- Sarsenbayeva, Assel (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
-
- Jernow, Henning (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
visa fler...
-
- Hetty, Susanne, PhD, 1979- (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
-
- Pereira, Maria J., 1981- (författare)
- Uppsala universitet,Klinisk diabetologi och metabolism
-
visa färre...
-
(creator_code:org_t)
- 2022-02-12
- 2022
- Engelska.
-
Ingår i: Laboratory medicine. - : Oxford University Press. - 0007-5027 .- 1943-7730. ; 53:5, s. E129-E133
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Objective: Research has shown that RNA extraction from adipose tissue (AT) is challenging because of high lipid content and low RNA quantity. We compared a traditional RNA extraction with a column-based method in human AT to evaluate RNA quantity and quality. Materials and Methods: Human subcutaneous AT (n = 9) was collected through needle biopsy, and RNA was extracted using the phenolchloroform traditional method and the RNeasy Lipid Tissue Mini Kit column-based method. The RNA quantity, quality, integrity, and expression of key AT genes were assessed. Results: We found that the RNA quantity and integrity were reduced by 40% and 15-20%, respectively, using the column-based method compared to the traditional method, but the findings were not statistically significant. The column-based method showed a higher 260/280 ratio (similar to 2.0) compared to the traditional method (similar to 1.8) (P <.05), suggesting lower amounts of contaminants. The expression of AT genes was comparable between methods. Conclusion: The traditional extraction method provides adequate RNA yield and integrity compared to the column-based method, which is an advantage when AT specimens are small.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- RNA isolation
- adipose tissue
- RNA quantity
- RNA purity
- traditional
- phenol-chloroform method
- column-based methods
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas