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Development and val...
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Dorlo, Thomas P C
(författare)
Development and validation of a quantitative assay for the measurement of miltefosine in human plasma by liquid chromatography-tandem mass spectrometry.
- Artikel/kapitelEngelska2008
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Elsevier BV,2008
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LIBRIS-ID:oai:DiVA.org:uu-494620
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-494620URI
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https://doi.org/10.1016/j.jchromb.2008.02.005DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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A sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for the quantification of miltefosine is presented. A 250 microL human EDTA plasma aliquot was spiked with miltefosine and extracted by a solid-phase extraction method. Separation was performed on a Gemini C18 column (150 mm x 2.0 mm I.D., 5 microm) using an alkaline eluent. Detection was performed by positive ion electrospray ionization followed by triple-quadrupole mass spectrometry. The assay has been validated for miltefosine from 4 to 2000 ng/mL using 250 microL human EDTA plasma samples. Results from the validation demonstrate that miltefosine can be accurately and precisely quantified in human plasma. At the lowest level, the intra-assay precision was lower than 10.7%, the inter-assay precision was 10.6% and accuracies were between 95.1 and 109%. This assay is successfully used in a clinical pharmacokinetic study with miltefosine.
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Hillebrand, Michel J X
(författare)
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Rosing, Hilde
(författare)
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Eggelte, Teunis A
(författare)
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de Vries, Peter J
(författare)
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Beijnen, Jos H
(författare)
Sammanhörande titlar
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Ingår i:Journal of chromatography. B: Elsevier BV865:1-2, s. 55-621570-02321873-376X
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